CAJ | 학술논문

副溶血弧菌是引起包括我国在内的世界各地沿海地区食物中毒的重要食源性致病菌,患者有典型的肠胃炎症状。及时准确地对食品中的副溶血弧菌进行检测是预防该菌引起的食物中毒的关键。分子生物学检测方法在副溶血弧菌检测中具有许多优势,现已得到广泛的应用。本文对PCR检测方法中的多重PCR、有扩增内标的PCR、实时荧光PCR(包括荧光染料法和荧光探针法)、基于DNA染料叠氮溴化乙锭和叠氮溴化丙锭的PCR、纳米粒子PCR、免疫捕获PCR、PCR-变性高效液相色谱、PCR-酶联免疫吸附等方法的国内外研究情况进行了综述,并对其检测效率、灵敏度、优点和缺点等进行了分析比较,环介导等温扩增(loop-mediated isothermal amplification, LAMP)--包括常规LAMP和原位LAMP因与PCR方法有相似之处，故一并进行了综述，为副溶血弧菌PCR检测方法的应用与开发提供参考。
부용혈호균시인기포괄아국재내적세계각지연해지구식물중독적중요식원성치병균,환자유전형적장위염증상。급시준학지대식품중적부용혈호균진행검측시예방해균인기적식물중독적관건。분자생물학검측방법재부용혈호균검측중구유허다우세,현이득도엄범적응용。본문대PCR검측방법중적다중PCR、유확증내표적PCR、실시형광PCR(포괄형광염료법화형광탐침법)、기우DNA염료첩담추화을정화첩담추화병정적PCR、납미입자PCR、면역포획PCR、PCR-변성고효액상색보、PCR-매련면역흡부등방법적국내외연구정황진행료종술,병대기검측효솔、령민도、우점화결점등진행료분석비교,배개도등온확증(loop-mediated isothermal amplification, LAMP)--포괄상규LAMP화원위LAMP인여PCR방법유상사지처，고일병진행료종술，위부용혈호균PCR검측방법적응용여개발제공삼고。
Vibrio paraheamolyticus is a leading cause of foodborn disease in the littoral areas worldwide, including China. The key to prevent foodborn disease caused by V. paraheamolyticus is rapid and accurate de-tection of this bacterium. The molecular biology method is commonly used now for its abundant advantages. The recent research progress both domestic and overseas was summarized in this paper and the detecting effi-ciency, sensitivity and the relative merits among multiplex PCR, internal amplification control PCR, real-time PCR, ethidium monoazide bromide PCR or propidium monoazide PCR, nanoparticle-based assisted PCR, im-munocapture PCR, PCR-denaturing high performance liquid chromatography and PCR-enzyme-linked immu-nosorbent assay were also compared and analyzed for the purpose of providing reference for the further re-searches and development. Loop-mediated isothermal amplification (LAMP), including conventional LAMP and in situ LAMP, similar to PCR, also is summarized.