CAJ | 학술논문

自山东省某疑似鸭肝炎发病鸭肝脏中分离到两株病毒，命名为TA1和TA2，分离毒分别回归3日龄雏鸭，可复制出鸭肝炎的典型症状和病理变化。利用鸭甲肝病毒1型（DHAV-1）和3型（DHAV-3）特异性引物进行RT-PCR 扩增，结果为阳性，经测序证实为DHAV-1和DHAV-3。分别扩增分离毒的VP1基因，经序列测定及遗传进化关系分析发现，分离毒TA1和DHAV-3毒株之间有较高的核苷酸序列相似性，与DHAV-3遗传距离最近，属于基因C型；分离毒TA2和DHAV-1毒株之间有较高的核苷酸序列相似性，与DHAV-1的遗传距离最近，属基因A型。
자산동성모의사압간염발병압간장중분리도량주병독，명명위TA1화TA2，분리독분별회귀3일령추압，가복제출압간염적전형증상화병리변화。이용압갑간병독1형（DHAV-1）화3형（DHAV-3）특이성인물진행RT-PCR 확증，결과위양성，경측서증실위DHAV-1화DHAV-3。분별확증분리독적VP1기인，경서렬측정급유전진화관계분석발현，분리독TA1화DHAV-3독주지간유교고적핵감산서렬상사성，여DHAV-3유전거리최근，속우기인C형；분리독TA2화DHAV-1독주지간유교고적핵감산서렬상사성，여DHAV-1적유전거리최근，속기인A형。
Two stains of duck hepatitis virus isolated from Shandong province were named TA1 and TA2.Clinical symptom and pathological lesion could be reproduced in 3-day ducklings challenged by the isolated strain.Two pairs of primers were designed and a reverse transcriptase polymerase chain reaction method was developed for strains TA1 and TA2. Nucleotide sequence identity of VP1 gene between strain TA1 and DHAV-3 strains was higher than DHAV-1.However,it was lower between strain TA2 and DHAV-3 than DHAV-1.Phylogenetic and evolutionary analysis showed that isolated strain TA1 belonged to genotype C, which were on different branches from strain TA2 belonged to genotype A.