热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2014年
7期
65-68
,共4页
廖韦卫%谢君锋%俸青%何洪良%唐君海
廖韋衛%謝君鋒%俸青%何洪良%唐君海
료위위%사군봉%봉청%하홍량%당군해
弄岗马兜铃%组织培养%生根诱导
弄崗馬兜鈴%組織培養%生根誘導
롱강마두령%조직배양%생근유도
Aristolochia longgonensis%tissue culture%rooting induction
对弄岗马兜铃组培苗进行生根诱导研究,以达到可移栽的目的。在1/2改良MS固体培养基(KH2PO4170 mg/L、蔗糖30 g/L, pH 5.8)中添加不同浓度的6-苄胺基腺嘌呤(6-BA)和吲哚丁酸(IBA)筛选最佳生根培养基配方。结果表明:在1/2改良MS固体培养基(蔗糖30 g/L, pH 5.8)加入1 mg/L的IBA具有较好的生根诱导作用,而6-BA对弄岗马兜铃组培苗不具有生根作用。
對弄崗馬兜鈴組培苗進行生根誘導研究,以達到可移栽的目的。在1/2改良MS固體培養基(KH2PO4170 mg/L、蔗糖30 g/L, pH 5.8)中添加不同濃度的6-芐胺基腺嘌呤(6-BA)和吲哚丁痠(IBA)篩選最佳生根培養基配方。結果錶明:在1/2改良MS固體培養基(蔗糖30 g/L, pH 5.8)加入1 mg/L的IBA具有較好的生根誘導作用,而6-BA對弄崗馬兜鈴組培苗不具有生根作用。
대롱강마두령조배묘진행생근유도연구,이체도가이재적목적。재1/2개량MS고체배양기(KH2PO4170 mg/L、자당30 g/L, pH 5.8)중첨가불동농도적6-변알기선표령(6-BA)화신타정산(IBA)사선최가생근배양기배방。결과표명:재1/2개량MS고체배양기(자당30 g/L, pH 5.8)가입1 mg/L적IBA구유교호적생근유도작용,이6-BA대롱강마두령조배묘불구유생근작용。
Aristolochia longgonensis is the unique medicinal plant in the Guangxi northern tropical mountainous area, the study has important significance in vitro rapid propagation and protection of it's germplasm resource, we study on the rooting induction of Aristolochia longgonensis tissue culture seedlings can achieve the purpose of transplantation. We used 1/2 modified MS culture medium (KH2PO4 170 mg/L, sucrose 30 g/L, pH 5.8) with different concentrations of 6-BA and IBA to select the best prescription of rooting medium. The result shows that the IBA has great effect on Aristolochia longgonensis tissue culture on rooting, the 1/2 modified MS culture medium (KH2PO4 170 mg/L, sucrose 30 g/L, pH 5.8) with 1 mg/L IBA has the best rooting induction effect, while 6-BA has no rooting effect.