热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2014年
7期
51-55
,共5页
巴西橡胶树%HbEC32%基因克隆%序列分析
巴西橡膠樹%HbEC32%基因剋隆%序列分析
파서상효수%HbEC32%기인극륭%서렬분석
Hevea brasiliensis%HbEC32%gene cloning%sequence analysis
以巴西橡胶树根为材料,利用RT-PCR技术从总RNA中捕获到一个β-木糖苷酶家族基因(β-xylosidase或β-D-xyloside xylohydrolase, EC 3.2.1.37), cDNA长度2880 bp,开放阅读框2319 bp,编码773个氨基酸,命名为HbEC32。在HbEC32氨基酸序列中具有一个较强的跨膜区,二级结构分析表明其属于混合型蛋白。HbEC32在N端前8～20氨基酸区域内为较强的疏水区,但整条多肽链表现为亲水性。对15个β-木糖苷酶蛋白进行系统进化分析发现,巴西橡胶树的HbEC32基因与蓖麻亲缘关系最近。
以巴西橡膠樹根為材料,利用RT-PCR技術從總RNA中捕穫到一箇β-木糖苷酶傢族基因(β-xylosidase或β-D-xyloside xylohydrolase, EC 3.2.1.37), cDNA長度2880 bp,開放閱讀框2319 bp,編碼773箇氨基痠,命名為HbEC32。在HbEC32氨基痠序列中具有一箇較彊的跨膜區,二級結構分析錶明其屬于混閤型蛋白。HbEC32在N耑前8～20氨基痠區域內為較彊的疏水區,但整條多肽鏈錶現為親水性。對15箇β-木糖苷酶蛋白進行繫統進化分析髮現,巴西橡膠樹的HbEC32基因與蓖痳親緣關繫最近。
이파서상효수근위재료,이용RT-PCR기술종총RNA중포획도일개β-목당감매가족기인(β-xylosidase혹β-D-xyloside xylohydrolase, EC 3.2.1.37), cDNA장도2880 bp,개방열독광2319 bp,편마773개안기산,명명위HbEC32。재HbEC32안기산서렬중구유일개교강적과막구,이급결구분석표명기속우혼합형단백。HbEC32재N단전8～20안기산구역내위교강적소수구,단정조다태련표현위친수성。대15개β-목당감매단백진행계통진화분석발현,파서상효수적HbEC32기인여비마친연관계최근。
A novel full-length β-D-xyloside xylohydrolase gene named HbEC32 was obtained by using reverse transcription-polymerase chain reaction (RT-PCR) from total RNA from the root of Hevea brasiliensis. HbEC32 was 2 880 bp long containing a 2 319-bp open reading frame (ORF), which encoded 773 amino acids. One strong transmembrane structure was predicted within HbEC32. The result of secondary structure predicted showed that the protein was classified as mixed type. There was a strong hydrophobic region in the N-terminal 8-20 amino acids of HbEC32, but the entire polypeptide chain was hydrophilic. The result of phylogenetic tree indicated that HbEC32 displayed a high degree of sequence similarity to RcEC32.
