CAJ | 학술논문

目的：探讨纳米Fe3 O4/C225复合物（简称复合物）对结直肠癌LoVo细胞放射增敏性的影响。方法采用MTT法观察不同浓度复合物（0、3?125、6?25、12?5、25、50、100mg/L）作用24h后的增殖抑制率，根据实验设计分为单纯照射组、C225＋照射组及复合物＋照射组，采用克隆形成实验检测3组经0、2、4、6、8和10Gy X线照射后的存活分数（ SF），流式细胞仪检测3组经4Gy X线照射24h后的细胞周期情况，采用Western blotting检测经4Gy X线照射24h后的表皮生长因子受体（ EGFR）蛋白水平及其相关信号通路中p-P38和p-Akt蛋白水平。结果在3?125～100mg/L范围内，随复合物浓度增加， LoVo细胞的增殖抑制率升高，0、3?125、6?25、12?5、25、50、100mg/L的增殖抑制率依次为0％、（8?950±0?086）％、（14?760±0?011）％、（29?860±0?001）％、（41?750±0?017）％、（59?770±0?010）％和（78?710±0?017）％，组间差异有统计学意义（P＜0?05）；复合物＋照射组的SF、S期细胞比例及p-Akt、EGFR蛋白水平均低于C225＋照射组和单纯照射组，G0/G1期、G2/M期细胞比例及p-P38蛋白水平均高于C225＋照射组和单纯照射组，以上差异均有统计学意义（ P＜0?05）。结论纳米Fe3 O4/C225复合物可抑制LoVo细胞增殖并具有放射增敏作用，可能与G0/G1期阻滞及EGFR相关信号通路受抑制有关。
목적：탐토납미Fe3 O4/C225복합물（간칭복합물）대결직장암LoVo세포방사증민성적영향。방법채용MTT법관찰불동농도복합물（0、3?125、6?25、12?5、25、50、100mg/L）작용24h후적증식억제솔，근거실험설계분위단순조사조、C225＋조사조급복합물＋조사조，채용극륭형성실험검측3조경0、2、4、6、8화10Gy X선조사후적존활분수（ SF），류식세포의검측3조경4Gy X선조사24h후적세포주기정황，채용Western blotting검측경4Gy X선조사24h후적표피생장인자수체（ EGFR）단백수평급기상관신호통로중p-P38화p-Akt단백수평。결과재3?125～100mg/L범위내，수복합물농도증가， LoVo세포적증식억제솔승고，0、3?125、6?25、12?5、25、50、100mg/L적증식억제솔의차위0％、（8?950±0?086）％、（14?760±0?011）％、（29?860±0?001）％、（41?750±0?017）％、（59?770±0?010）％화（78?710±0?017）％，조간차이유통계학의의（P＜0?05）；복합물＋조사조적SF、S기세포비례급p-Akt、EGFR단백수평균저우C225＋조사조화단순조사조，G0/G1기、G2/M기세포비례급p-P38단백수평균고우C225＋조사조화단순조사조，이상차이균유통계학의의（ P＜0?05）。결론납미Fe3 O4/C225복합물가억제LoVo세포증식병구유방사증민작용，가능여G0/G1기조체급EGFR상관신호통로수억제유관。
Objective To investigate the radiosensitivity enhancement and possible mechanism of nano Fe3 O4/C225 complex on human colorectal cancer LoVo cells. Methods In vitro inhibition rates of cell proliferation at 24h after treatment with different con-centrations (0, 3?125, 6?25, 12?5, 25, 50, 100 mg/mL) of nano Fe3O4/C225 complex were determined by MTT. According to the experimental protocol, the below experiments were carried out in irradiation group, C225+irradiation group and complex+irradiation group. The colony formation assay was employed to observe the surviving fraction ( SF) of three groups after X-ray irradiation of 0, 2, 4, 6, 8, 10Gy. The cell cycle of three groups at 24h after X-ray irradiation of 4Gy was measured by flow cytometry. Western blotting was employed to measure the protein level of epidermal growth factor receptor ( EGFR) and p-P38, p-Akt in the related of signaling pathway. Results The proliferation inhibition rate of LoVo cells gradually increased with the increasing concentration of Fe3 O4/C225 complex ranging from 3?125 to 100 mg/L. The proliferation inhibition rates were 0%, (8?950± 0?086)%, (14?760± 0?011)%, (29?860± 0?001)%, (41?750± 0?017)%, (59?770± 0?010)% and (78?710± 0?017)% at the concentrations of 0, 3?125, 6?25, 12?5, 25, 50, 100mg/mL with statistically significant differences ( P<0?05) . Compared with irradiation group and C225+irradiation group, there were lower SF, percentage of S cells and protein levels of p-Akt, EGFR and higher percentages of G0/G1 and G2/M and protein level of p-P38 in complex+irradiation group with statistically significant differences (P<0?05). Conclusion Nano Fe3O4/C225 complex could inhibit the proliferation of LoVo cells with radiosensitizing effect possibly via the arrest at the G0/G1 phase and in-hibition of EGFR signaling pathways.