激光杂志
激光雜誌
격광잡지
LASER JOURNAL
2014年
7期
123-124
,共2页
李嫄%胡江波%张敉%张景勋
李嫄%鬍江波%張敉%張景勛
리원%호강파%장미%장경훈
姜黄素%脂质体%壳聚糖%包封率
薑黃素%脂質體%殼聚糖%包封率
강황소%지질체%각취당%포봉솔
Curcumin%Liposomes%Chitosan%Entrapment efficiency
目的：建立壳聚糖包衣姜黄素脂质体包封率的测定方法。方法：采用紫外分光光度法，在426 nm处测定吸光度值，计算壳聚糖包衣姜黄素脂质体中包封的药物量，由此计算出包封率。结果：姜黄素在426 nm处有最大吸收，浓度在0.5～8 mg/mL时与吸光度呈良好的线性关系，回归方程为A=0.1440C-0.008，R=0.9998（n=3）；平均加样回收率为99.96%，RSD为0.29%。结论：本方法操作简单、快捷，能够应用于测定壳聚糖包衣姜黄素脂质体的包封率。
目的：建立殼聚糖包衣薑黃素脂質體包封率的測定方法。方法：採用紫外分光光度法，在426 nm處測定吸光度值，計算殼聚糖包衣薑黃素脂質體中包封的藥物量，由此計算齣包封率。結果：薑黃素在426 nm處有最大吸收，濃度在0.5～8 mg/mL時與吸光度呈良好的線性關繫，迴歸方程為A=0.1440C-0.008，R=0.9998（n=3）；平均加樣迴收率為99.96%，RSD為0.29%。結論：本方法操作簡單、快捷，能夠應用于測定殼聚糖包衣薑黃素脂質體的包封率。
목적：건립각취당포의강황소지질체포봉솔적측정방법。방법：채용자외분광광도법，재426 nm처측정흡광도치，계산각취당포의강황소지질체중포봉적약물량，유차계산출포봉솔。결과：강황소재426 nm처유최대흡수，농도재0.5～8 mg/mL시여흡광도정량호적선성관계，회귀방정위A=0.1440C-0.008，R=0.9998（n=3）；평균가양회수솔위99.96%，RSD위0.29%。결론：본방법조작간단、쾌첩，능구응용우측정각취당포의강황소지질체적포봉솔。
Objective:To establish the method determination of the entrapment efficiency of chitosan coated cur-cumin liposomes. Method:Entrapment efficiency was determined at the wavelength of 426 nm by UV-visible spec-trophotometry. Result:The curcumin had a good liner relationship range from 0.5~8 μg/mL. The regression equa-tion was as follows:A= 0.1440C- 0.008,R=0.9998(n=3). The average recovery rate was 99.96%(n=9),RSD =0.29%. Conclusion:The method was simple and rapid,which can be used for the determination of entrapment effi-ciency of chitosan coated curcumin liposomes.
