化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2014年
7期
27-29,33
,共4页
巩育军%牛盛童%黄学锋%王键
鞏育軍%牛盛童%黃學鋒%王鍵
공육군%우성동%황학봉%왕건
离子液体%双水相体系%山楂%黄酮%多糖
離子液體%雙水相體繫%山楂%黃酮%多糖
리자액체%쌍수상체계%산사%황동%다당
ionic liquid%aqueous two-phase system%hawthorn%flavonoids%polysaccharide
采用分光光度法研究了山楂黄酮和多糖在[Bmim]BF4/（NH4）2 SO4双水相体系的分配行为，探讨了离子液体浓度、（NH4）2 SO4浓度、山楂用量和超声时间等因素对山楂黄酮和多糖萃取率的影响。确定最佳萃取条件为：离子液体[Bmim]BF4浓度0.26～0.30 g·mL-1，（NH4）2 SO4浓度0.08～0.10 g·mL-1，山楂用量0.14～0.17 g，超声时间15～20 min，在此优化条件下，双水相上相中黄酮的萃取率为86.4％～96.0％、下相中多糖的萃取率为75.2％～76.0％。
採用分光光度法研究瞭山楂黃酮和多糖在[Bmim]BF4/（NH4）2 SO4雙水相體繫的分配行為，探討瞭離子液體濃度、（NH4）2 SO4濃度、山楂用量和超聲時間等因素對山楂黃酮和多糖萃取率的影響。確定最佳萃取條件為：離子液體[Bmim]BF4濃度0.26～0.30 g·mL-1，（NH4）2 SO4濃度0.08～0.10 g·mL-1，山楂用量0.14～0.17 g，超聲時間15～20 min，在此優化條件下，雙水相上相中黃酮的萃取率為86.4％～96.0％、下相中多糖的萃取率為75.2％～76.0％。
채용분광광도법연구료산사황동화다당재[Bmim]BF4/（NH4）2 SO4쌍수상체계적분배행위，탐토료리자액체농도、（NH4）2 SO4농도、산사용량화초성시간등인소대산사황동화다당췌취솔적영향。학정최가췌취조건위：리자액체[Bmim]BF4농도0.26～0.30 g·mL-1，（NH4）2 SO4농도0.08～0.10 g·mL-1，산사용량0.14～0.17 g，초성시간15～20 min，재차우화조건하，쌍수상상상중황동적췌취솔위86.4％～96.0％、하상중다당적췌취솔위75.2％～76.0％。
Partition behaviors of hawthorn flavonoids and polysaccharide in ionic liquid aqueous two-phase system(ILATPS)were studied by spectrophotometry.Effects of concentration of ionic liquid and ammonium sulfate,dosage of hawthorn and ultrasonic time on the extraction rate of hawthorn flavonoids and polysaccharide were investigated.The optimal extraction conditions were as follows:concentration of ionic liquid [Bmim]BF4 was 0.26~0.30 g·mL-1,concentration of ammonium sulfate was 0.08~0.10 g·mL-1,dosage of hawthorn was 0.14~0.17 g,ultrasonic time was 15~20 min.Under these conditions,extraction rate of hawthorn fla-vonoids was 86.4%~96.0% in the up phase,and extraction rate of polysaccharide was 75.2%~76.0% in the down phase.
