化学与生物工程
化學與生物工程
화학여생물공정
CHEMISTRY & BIOENGINEERING
2014年
7期
9-12
,共4页
靖钰%张存超%付托%蒋成%张学光%寇庚
靖鈺%張存超%付託%蔣成%張學光%寇庚
정옥%장존초%부탁%장성%장학광%구경
CHO细胞%人苹果酸脱氢酶Ⅱ%补料分批培养%凋亡%活性氧
CHO細胞%人蘋果痠脫氫酶Ⅱ%補料分批培養%凋亡%活性氧
CHO세포%인평과산탈경매Ⅱ%보료분비배양%조망%활성양
Chinese hamster ovary cells%human malate dehydrogenase Ⅱ%fed-batch culture%apoptosis%reac-tive oxygen species
构建人苹果酸脱氢酶Ⅱ（hMDHⅡ）过表达的重组 CHO细胞，并研究 hMDHⅡ过表达对过氧化氢压力下CHO细胞凋亡的影响。结果显示，在0.1 mmol·L-1过氧化氢压力下，hMDHⅡ过表达明显提高了补料分批培养后期细胞活率。线粒体膜电位及活性氧检测显示，hMDHⅡ过表达后线粒体膜电位显著提高、活性氧显著降低，表明 hMDHⅡ过表达抑制细胞凋亡可能是通过减少细胞内活性氧而实现的。
構建人蘋果痠脫氫酶Ⅱ（hMDHⅡ）過錶達的重組 CHO細胞，併研究 hMDHⅡ過錶達對過氧化氫壓力下CHO細胞凋亡的影響。結果顯示，在0.1 mmol·L-1過氧化氫壓力下，hMDHⅡ過錶達明顯提高瞭補料分批培養後期細胞活率。線粒體膜電位及活性氧檢測顯示，hMDHⅡ過錶達後線粒體膜電位顯著提高、活性氧顯著降低，錶明 hMDHⅡ過錶達抑製細胞凋亡可能是通過減少細胞內活性氧而實現的。
구건인평과산탈경매Ⅱ（hMDHⅡ）과표체적중조 CHO세포，병연구 hMDHⅡ과표체대과양화경압력하CHO세포조망적영향。결과현시，재0.1 mmol·L-1과양화경압력하，hMDHⅡ과표체명현제고료보료분비배양후기세포활솔。선립체막전위급활성양검측현시，hMDHⅡ과표체후선립체막전위현저제고、활성양현저강저，표명 hMDHⅡ과표체억제세포조망가능시통과감소세포내활성양이실현적。
In the study,we constructed recombinant CHO cells over-expressing human malate dehydrogen-ase Ⅱ(hMDHⅡ),and investigated its impact on apoptosis under hydrogen peroxide treatment.The results showed that hMDHⅡ over-expression improves the cell survival rate in fed-batch culture under 0.1 mmol·L-1 hydrogen peroxide treatment.Analysis on mitochondrial membrane potential and reactive oxygen species(ROS) showed that,after hMDHⅡ over-expression mitochondrial membrane potential increases significantly,which means reduction in apoptosis,and ROS decreases.This suggests that inhibition of apoptosis by over-expression of hMDHⅡ may due to decrease in ROS.This study will further the understanding on ROS-induced apoptosis, and also provide suggestions for the construction of cell line with the property of anti-apoptosis.
