CAJ | 학술논문

目的：探讨 HBV前C区1896变异与乙型肝炎病毒相关性肝癌患者外周血T 淋巴细胞亚群之间的关系。方法采用特异性引物PCR检测120例 HBV相关性肝癌患者前C区1896变异，并用流式细胞仪检测外周血T 淋巴细胞亚群的变化。以20名健康献血者作为健康对照组。结果120例乙肝相关性肝癌中前C区1896变异检出率为65％；其前C区1896变异构成比在≥50岁与＜50岁患者间的差异无统计学意义（χ2＝0．024，P＝0．878）；HBV前C区1896突变株感染和野生株感染的肝癌患者性别差异无统计学意义（χ2＝0．899，P＝0．343），前 C 区1896突变株感染肝癌患者HBV DNA定量明显高于野生株感染的肝癌患者（t＝7．01，P＜0．05）；HBV相关 HCC组CD3＋、CD4＋、CD8＋T淋巴细胞百分数以及CD4＋/CD8＋比值明显低于健康对照组（P＜0．01），而 CD8＋T 淋巴细胞百分数明显高于正常对照组（P＜0．01）；前C区1896突变株感染肝癌患者CD3＋、CD4＋、CD8＋T淋巴细胞百分数以及CD4＋/CD8＋比值明显低于野生株感染的肝癌患者（P＜0．01），而CD8＋T淋巴细胞百分数明显高于野生株感染 HCC 患者（P＜0．01）。结论 HBV 前C 区1896突变株感染的乙肝相关性肝癌患者较 HBV野生株感染的肝癌患者存在更严重的T 淋巴细胞亚群失衡，这种紊乱可能参与了 HBV前C区1896突变株感染导致的肝癌过程。
목적：탐토 HBV전C구1896변이여을형간염병독상관성간암환자외주혈T 림파세포아군지간적관계。방법채용특이성인물PCR검측120례 HBV상관성간암환자전C구1896변이，병용류식세포의검측외주혈T 림파세포아군적변화。이20명건강헌혈자작위건강대조조。결과120례을간상관성간암중전C구1896변이검출솔위65％；기전C구1896변이구성비재≥50세여＜50세환자간적차이무통계학의의（χ2＝0．024，P＝0．878）；HBV전C구1896돌변주감염화야생주감염적간암환자성별차이무통계학의의（χ2＝0．899，P＝0．343），전 C 구1896돌변주감염간암환자HBV DNA정량명현고우야생주감염적간암환자（t＝7．01，P＜0．05）；HBV상관 HCC조CD3＋、CD4＋、CD8＋T림파세포백분수이급CD4＋/CD8＋비치명현저우건강대조조（P＜0．01），이 CD8＋T 림파세포백분수명현고우정상대조조（P＜0．01）；전C구1896돌변주감염간암환자CD3＋、CD4＋、CD8＋T림파세포백분수이급CD4＋/CD8＋비치명현저우야생주감염적간암환자（P＜0．01），이CD8＋T림파세포백분수명현고우야생주감염 HCC 환자（P＜0．01）。결론 HBV 전C 구1896돌변주감염적을간상관성간암환자교 HBV야생주감염적간암환자존재경엄중적T 림파세포아군실형，저충문란가능삼여료 HBV전C구1896돌변주감염도치적간암과정。
Objective To investigate the relationship of hepatitis B Virus (HBV)preC region 1896 mutation with peripheral blood T lymphocyte subsets of hepatitis B related hepatocellular carcinoma(HCC).Methods In 120 patients with hepatitis B related HCC,the specific primers of polymerase chain reaction(PCR)was used to detect the (HBV)preC region 1 896 mutation,and the T lymphocyte subsets in peripheral blood were detected by flow cytometry.A total of 20 healthy subjects were enrolled as healthy control group.Results In 120 hepatitis B related HCC,the rate of HBV preC region 1896 mutation was 65% ;No significant difference was found in the proportion of HBV preC 1896 mutation between ≥50 years and <50 years(χ2=0.024,P=0.878);None of the HCC With HBV preC 1896 mutation group showed a significantly different sex from that detected in HCC With wild-type strain group (χ2=0 .899 ,P=0 .343 );The HBV-DNA loading in HCC With HBV preC 1896 mutation was higher than that in HCC With wild-type strain(t=7.01,P<0.05).In hepatitis B related HCC group ,the percentage of CD3+ T cell,the percentage of CD3+ T cell,the percentage of CD4+ T cell and CD4+T/CD8+ ratio were lower than those in the healthy control group(P<0.01),but the percentage of CD8+ T cell was higher than those in the healthy control group (P<0.01 );Compared to the hepatitis B related HCC With wild-type strain group ,the percentage of CD3+ T cell,the percentage of CD4+ T cell and CD4+T/CD8+ ratio in hepatitis B related HCC With HBV preC region 1896 mutation had significiant decrease (P <0.01 ),but the percentage of CD8+ T cell had significiant increase(P<0.01).Conclusion In hepatitis B related HCC With HBV preC 1896 mutation,the imbalance of T lymphocyte subsets is more serious than that of hepatitis B related HCC With wild-type strain.The imbalance may participate in the pathogenesis of HCC caused by the HBV preC region 1 896 mutation.