CAJ | 학술논문

以十六烷基溴化铵(CTAB)为结构导向剂,正硅酸乙酯(TEOS)为硅源,在碱性环境下经过自组装过程对单分散性磁性 Fe3 O4纳米粒子进行包覆,制备出磁性硅基介孔纳米粒子 Fe3 O4@ SiO2.结合 X 射线衍射、傅里叶变换红外光谱(FTIR)、透射电子显微镜(TEM)以及氮气吸附-脱附等技术对 Fe3 O4@ SiO2粒子进行表征.结果表明 Fe3 O4@ SiO2纳米粒子具有球形形貌,平均直径约为150 nm,蠕虫状介孔结构,比表面积为932 m2/ g,孔径为2.5 nm 且分布较均匀,包覆后 Fe3 O4的结构得以保持,同时材料具有很好的磁响应能力.以抗癌药紫杉醇(Paelitaxel, TXL)为模型药物进行负载,实验结果表明, Fe3 O4@ SiO2对 TXL 的负载能力为80 mg/ g, TXL-Fe3 O4@ SiO2对 TXL 的缓释时间持续120 h 以上,累积释放量达到30 mg/ g.通过噻唑蓝比色(MTT)法测量了 TXL-Fe3 O4@ SiO2粒子对体外培养的 HeLa 细胞的细胞毒性,与相同浓度的 TXL 相比, TXL-Fe3 O4@ SiO2对 HeLa 细胞的抑制率明显增高.
이십륙완기추화안(CTAB)위결구도향제,정규산을지(TEOS)위규원,재감성배경하경과자조장과정대단분산성자성 Fe3 O4납미입자진행포복,제비출자성규기개공납미입자 Fe3 O4@ SiO2.결합 X 사선연사、부리협변환홍외광보(FTIR)、투사전자현미경(TEM)이급담기흡부-탈부등기술대 Fe3 O4@ SiO2입자진행표정.결과표명 Fe3 O4@ SiO2납미입자구유구형형모,평균직경약위150 nm,연충상개공결구,비표면적위932 m2/ g,공경위2.5 nm 차분포교균균,포복후 Fe3 O4적결구득이보지,동시재료구유흔호적자향응능력.이항암약자삼순(Paelitaxel, TXL)위모형약물진행부재,실험결과표명, Fe3 O4@ SiO2대 TXL 적부재능력위80 mg/ g, TXL-Fe3 O4@ SiO2대 TXL 적완석시간지속120 h 이상,루적석방량체도30 mg/ g.통과새서람비색(MTT)법측량료 TXL-Fe3 O4@ SiO2입자대체외배양적 HeLa 세포적세포독성,여상동농도적 TXL 상비, TXL-Fe3 O4@ SiO2대 HeLa 세포적억제솔명현증고.
Magnetic Fe3 O4@ SiO2 mesoporous silica nanoparticles(MSNs) were synthesized with Fe3 O4 nano-particles as crystal core, cetyltrimethylammium bromide( CTAB) as structural directional agent through the hydrolysis of tetraethoxysilane(TEOS) and self-assembly procedure under the basic conditions. The synthe-sized MSNs were characterized via the XRD, FTIR, TEM and N2 adsorptiom-desorption. The results show that these MSNs have high sepcific surface area of 932 cm2 / g, particles size of 150 nm and typical hexagonal packing mesoporous structure. Simultaneously, all Fe3 O4 nanoparticles are perfectly embedded in silica bulk. As a TXL carrier, Fe3 O4 @ SiO2 exhibits good adsorption with the maximum adsorption capacity of 80 mg / g and TXL-Fe3 O4@ SiO2 has a more than 120 h sustained release for TXL with accumulative quantity of 30 mg /g. While the release behaviour is controlled by various pH values. Higher the pH is, larger the accumulative release is. Experiments of cytotoxicity on HeLa cells indicate that the Fe3 O4@ SiO2 particles exhibit excellent biocompatibility. TXL-Fe3 O4@ SiO2 shows significant inhibition effect on HeLa cells than bare TXL under the same drug concentration.