CAJ | 학술논문

为研究关白附多糖及其硫酸酯的经典途径抗补体活性,以关白附[Aconitum coreanum(Lévl.) Raipaics]为原料,经水提醇沉、 DE-32、 Superdex-200和 Superdex-75凝胶柱分离纯化,得到1个均一的中性多糖KMPS-2A.采用高效凝胶渗透色谱法、甲基化、核磁共振和红外光谱等手段对 KMPS-2A 的结构进行了鉴定；采用氯磺酸吡啶法制备了多糖硫酸酯,并测定了多糖及硫酸酯的抗补体活性.结果表明, KMPS-2A 的平均相对分子量为6.76×105,结构为α-1,6-D-Glc 链接的线性多糖；在氯磺酸与吡啶的体积比为1.75：1.0时制备的多糖硫酸酯1.75B 的取代度最高为1.79.碳核磁谱分析结果表明,硫酸基团先后取代 C2, C3及 C4位.该多糖硫酸酯的抗补体活性与其硫酸基团取代度呈现一定的相关性,多糖硫酸酯1.75B 的经典途径抗补体活性优于阳性对照药肝素,表明其具有开发成为补体抑制剂的潜力.
위연구관백부다당급기류산지적경전도경항보체활성,이관백부[Aconitum coreanum(Lévl.) Raipaics]위원료,경수제순침、 DE-32、 Superdex-200화 Superdex-75응효주분리순화,득도1개균일적중성다당KMPS-2A.채용고효응효삼투색보법、갑기화、핵자공진화홍외광보등수단대 KMPS-2A 적결구진행료감정；채용록광산필정법제비료다당류산지,병측정료다당급류산지적항보체활성.결과표명, KMPS-2A 적평균상대분자량위6.76×105,결구위α-1,6-D-Glc 련접적선성다당；재록광산여필정적체적비위1.75：1.0시제비적다당류산지1.75B 적취대도최고위1.79.탄핵자보분석결과표명,류산기단선후취대 C2, C3급 C4위.해다당류산지적항보체활성여기류산기단취대도정현일정적상관성,다당류산지1.75B 적경전도경항보체활성우우양성대조약간소,표명기구유개발성위보체억제제적잠력.
In order to study anti-complement activity in the classical pathway of Korean Mondshood root and its sulfated polysaccharides, a homogeneous polysaccharide(KMPS-2A) was gained from the Korean Monds-hood root[Aconitum coreanum(Lévl. ) Raipaics] by water extract and alcohol precipitate via DE-32, Super-dex-200 and Superdex-75 gel filtration. Its structure was investigated by high performance gel permeation chro-matography, methylation analysis, NMR and IR spectrometry. The results showed that the average molecular weights(Mw ) of the KMPS-2A was 676000 and it was consisted of a repeating structure as [α-1,6-D-Glc] n . Sulfated derivatives were gained by chlorosulfonic acid-pyridine method and it was found that the sulfated de-rivatives had the highest degree of substitution(1. 79) when the volume ratio of chlorosulfonic acid and pyri-dine was 1. 75 : 1. 0. It was shown that sulfated polysaccharide 1. 75B had a better anti-complementary activi-ty. C2, C3 and C4 were substituted by sulfate groups. A certain correlation was presented between the degree of substitution and the anticomplementary activity of sulfated derivatives. Anticomplementary activity of sulfated derivative 1. 75B was better than the heparin group in the classical pathway, which suggested the po-tential activity of the complement inhibitors.