CAJ | 학술논문

将1,4-二硫苏糖醇(DTT)自组装在100 nm 厚的平整金膜表面,形成 DTT 膜修饰金平板电极(GPE),构建了一种新颖的简单、快速测定汞离子的选择性电极分析方法.通过电化学交流阻抗和循环伏安法探讨了该电极的响应原理,即固定在 Au 表面的 DTT 通过另一端的巯基与汞离子发生强配位作用而吸附结合带正电荷的汞离子,引起电极表面膜电位的变化,从而选择性地识别汞离子.实验结果表明,该电极在 pH =6.0的 Tris-HCl 缓冲溶液中对汞离子有良好的电位响应性能,其线性范围为1.0×10-8~1.0×10-3 mol/ L,能斯特响应斜率为(29.62±0.2) mV/-pc(25℃),检出限为5.1×10-9 mol/ L.该汞离子检测电极的响应时间仅为20 s,且有较好的重现性和稳定性.通过测定各种离子的选择性系数,发现 Cu2+, Fe2+, Na+, K+, Mg2+, Ba2+, Ca2+, Zn2+, Sn2+, Pb2+, Ag+, Al3+, Fe3+, Ni2+, NO-2, IO-3, BrO-3和 ClO-3等离子不干扰该电极对汞离子的检测.此外,将该电极用于实际水样中微量汞离子含量的测定,结果与双硫腙分光光度方法一致,且回收率为98.20%~101.75%.
장1,4-이류소당순(DTT)자조장재100 nm 후적평정금막표면,형성 DTT 막수식금평판전겁(GPE),구건료일충신영적간단、쾌속측정홍리자적선택성전겁분석방법.통과전화학교류조항화순배복안법탐토료해전겁적향응원리,즉고정재 Au 표면적 DTT 통과령일단적구기여홍리자발생강배위작용이흡부결합대정전하적홍리자,인기전겁표면막전위적변화,종이선택성지식별홍리자.실험결과표명,해전겁재 pH =6.0적 Tris-HCl 완충용액중대홍리자유량호적전위향응성능,기선성범위위1.0×10-8~1.0×10-3 mol/ L,능사특향응사솔위(29.62±0.2) mV/-pc(25℃),검출한위5.1×10-9 mol/ L.해홍리자검측전겁적향응시간부위20 s,차유교호적중현성화은정성.통과측정각충리자적선택성계수,발현 Cu2+, Fe2+, Na+, K+, Mg2+, Ba2+, Ca2+, Zn2+, Sn2+, Pb2+, Ag+, Al3+, Fe3+, Ni2+, NO-2, IO-3, BrO-3화 ClO-3등리자불간우해전겁대홍리자적검측.차외,장해전겁용우실제수양중미량홍리자함량적측정,결과여쌍류종분광광도방법일치,차회수솔위98.20%~101.75%.
A simply gold plate electrode(GPE) based on 1,4-dithiothreitol(DTT), which was self-assembled on a surface of flat gold film with a thickness of 100 nm, was developed to construct a novel selective electrode method for rapid detection of mercury ion. Through electrochemical impedance analysis and cyclic voltamme-tric method, the response mechanism of the electrode for selective recognition of Hg2+ was investigated, that the other terminal sulfhydryl group of DTT binding to Au surface can coordinate with Hg2+ due to their strong complexing interaction, resulting in change of membrane potential of the electrode surface. The proposed elec-trode possesses good potential performance responding to Hg2+ with a linear range of 1. 0 ×10-8-1. 0 ×10-3 mol/ L, a Nernst slope of (29. 62±0. 2) mV/ -pc(25 ℃), and a detection limit of 5. 1×10-9 mol/ L in Tris-HCl buffer solution(pH=6. 0). The electrode has short response time(20 s), good reproducibility and stabili-ty. No interference can be observed from most common ions like Cu2+, Fe2+, Na+, K+, Mg2+, Ba2+, Ca2+, Zn2+, Sn2+, Pb2+, Ag+, Al3+, Fe3+, Ni2+, NO-2 , IO-3 , BrO-3 and ClO-3 . Compared with spectrophotometric method with dithizone, the proposed electrode can be well utilized to the determination of trace amount of Hg2+in real water samples with a recovery rate of 98. 20% -101. 75% , showing promising application in environ-mental and other fields.