CAJ | 학술논문

通过自组装辅助的一步法制备了具有温度和 pH 双重响应性的荧光纳米凝胶(FNG)。首先设计制备了一种水溶性含双键的荧光单体5-丙烯酰胺荧光素(5-AAF),在水溶性纤维素醚---羟丙基纤维素(HPC)主链上引发5-AAF 的接枝共聚,同时由于5-AAF 的疏水作用力诱导共聚物发生自组装,并通过双官能团交联剂亚甲基二丙烯酰胺(MBA)的加入使自组装纳米聚集体交联,从而一步制得具有环境响应性的 FNG,该过程在水相中进行,具有高效、“绿色”的优点。研究结果表明,改变合成过程中 HPC 的分子量可调控所得FNG 的环境响应性。对 FNG 环境响应性的研究表明, FNG 链段上的亲疏水基团及与水分子间的氢键作用是影响凝胶温度响应性的主要因素。此外, FNG 的荧光在中性及碱性溶液中显著加强,在酸性溶液中迅速猝灭。由于 FNG 的荧光信号对温度和 pH 的显著敏感性,且具有较低的细胞毒性,因此在荧光标记生物检测及生物微环境的温度/ pH 检测等领域具有广泛的应用前景。
통과자조장보조적일보법제비료구유온도화 pH 쌍중향응성적형광납미응효(FNG)。수선설계제비료일충수용성함쌍건적형광단체5-병희선알형광소(5-AAF),재수용성섬유소미---간병기섬유소(HPC)주련상인발5-AAF 적접지공취,동시유우5-AAF 적소수작용력유도공취물발생자조장,병통과쌍관능단교련제아갑기이병희선알(MBA)적가입사자조장납미취집체교련,종이일보제득구유배경향응성적 FNG,해과정재수상중진행,구유고효、“록색”적우점。연구결과표명,개변합성과정중 HPC 적분자량가조공소득FNG 적배경향응성。대 FNG 배경향응성적연구표명, FNG 련단상적친소수기단급여수분자간적경건작용시영향응효온도향응성적주요인소。차외, FNG 적형광재중성급감성용액중현저가강,재산성용액중신속졸멸。유우 FNG 적형광신호대온도화 pH 적현저민감성,차구유교저적세포독성,인차재형광표기생물검측급생물미배경적온도/ pH 검측등영역구유엄범적응용전경。
Temperature / pH dual-sensitive fluorescence nanogels(FNGs) were synthesized through a self-as-sembly assisted(SAA) “one-step” method. A hydrophilic fluorescence monomer 5-acrylamide fluorescein(5-AAF) has been fabricated, on this basis, graft copolymerization of 5-AAF from the backbone of hydroxypropyl cellulose(HPC) was initiated using ceric ammonium nitrate(CAN), the self-assembly of 5-AAF graft chains induced by both hydrogen bond and the hydrophobic interaction of fluorescence group proceeded with the poly-merization of 5-AAF. Furthermore, N,N’-methylenebisacrylamide(MBA) was applied as crosslinker. As a re-sult, FNGs were synthesized efficiently and environment-friendly with stable temperature / pH sensitivity prop-erties. The environment-sensitivity of resultant FNGs can be tuned by changing molecular weight of HPC, the existence of hydrophobic / hydrophilic group on FNGs as well as the hydrogen bond contributed to FNGs’ tem-perature-sensitivity. Moreover, FNGs displayed impressive pH-sensitivity under various pH values, the fluo-rescence intensity can be rapidly enhanced at neutral and alkalic environment, while it quenches at acidic en-vironment. The influence of pH on fluorescence intensity can be reversed without any other recovery process. The cytotoxicity of FNG was preliminarily studied, the results revealed good biocompatibility of FNGs. The temperature / pH responsivity of the FNGs’ fluorescence are of great potential in biomedical detection, such as fluorescence labeling, or temperature / pH sensor for micro-environment.