CAJ | 학술논문

以大白菜吉红82、#534DH群体及#438群体为试材，研究了利用细胞破碎仪机械提取小孢子与人工挤压提取小孢子对大白菜游离小孢子活力及提取量的影响。结果表明：机械提取替代人工挤压可在短时间内同时提取多份样品，并可快速调整小孢子的培养浓度。收集40个适期花蕾于5mL离心管内，3000r·min-1破碎10s，将提取的小孢子定容于25mLNLN-13培养基，可确保小孢子浓度在适宜培养范围内，并成功诱导胚胎发生。
이대백채길홍82、#534DH군체급#438군체위시재，연구료이용세포파쇄의궤계제취소포자여인공제압제취소포자대대백채유리소포자활력급제취량적영향。결과표명：궤계제취체대인공제압가재단시간내동시제취다빈양품，병가쾌속조정소포자적배양농도。수집40개괄기화뢰우5mL리심관내，3000r·min-1파쇄10s，장제취적소포자정용우25mLNLN-13배양기，가학보소포자농도재괄의배양범위내，병성공유도배태발생。
Taking Chinese cabbage〔Brassica campestris L. ssp. pekinensis(Lour)Olsson〕‘Jihong 82’,some of #534DH and #438 population as testing material,studies the effect of mechanical extraction and artificial extrusion on micro-spore viability and extraction quantity.The result indicates that mechanical extraction can replace the artificial one,get multiple samples during short period of time,and rapidly adjust the concentration of microspores culture.40 suitable buds are collected in 5 mL centrilfuge tubes,3 000 r·min-1 micro-spores gained by 10 s smashing extraction are isolated in 25 mL NLN-13 medium.Thus,micro-spore concentration is insured in suitable culture scope,and successfully induced to embryogenesis.