CAJ | 학술논문

利用凝胶层析技术从泥蚶血细胞裂解物中分离纯化得到两种血红蛋白，回收率为74．3％，并通过 Tricine-SDS-PAGE 和质谱技术鉴定为 Tg-HbⅠ（同源二聚体）和 Tg-HbⅡ（异源四聚体）。分别通过琼脂扩散法和分光光度法测其抗菌活性和过氧化物酶活性，结果发现，两种 Tg-Hb 对大肠杆菌和恶臭假单胞杆菌均有抗菌活性，Tg-HbⅡ对于恶臭假单胞菌和大肠杆菌的 MIC 值分别为0．063 mg／mL和0．13 mg／mL，Tg-HbⅠ对两种菌的 MIC 均为0．048 mg／mL；两种 Tg-Hb 均具有过氧化物酶活性，能催化愈创木酚、邻苯二酚、对苯二酚、苯酚、左旋多巴等多种酚类物质。在 Tg-Hb 中添加 GSH 后，两种 Tg-Hb 均不再对恶臭假单胞杆菌具有抗菌活性。可见，两种 Tg-Hb 对恶臭假单胞杆菌等的抗菌活性均是通过其过氧化物酶活性发挥作用的。
이용응효층석기술종니감혈세포렬해물중분리순화득도량충혈홍단백，회수솔위74．3％，병통과 Tricine-SDS-PAGE 화질보기술감정위 Tg-HbⅠ（동원이취체）화 Tg-HbⅡ（이원사취체）。분별통과경지확산법화분광광도법측기항균활성화과양화물매활성，결과발현，량충 Tg-Hb 대대장간균화악취가단포간균균유항균활성，Tg-HbⅡ대우악취가단포균화대장간균적 MIC 치분별위0．063 mg／mL화0．13 mg／mL，Tg-HbⅠ대량충균적 MIC 균위0．048 mg／mL；량충 Tg-Hb 균구유과양화물매활성，능최화유창목분、린분이분、대분이분、분분、좌선다파등다충분류물질。재 Tg-Hb 중첨가 GSH 후，량충 Tg-Hb 균불재대악취가단포간균구유항균활성。가견，량충 Tg-Hb 대악취가단포간균등적항균활성균시통과기과양화물매활성발휘작용적。
Two kinds of hemoglobins were purified by gel chromatography with a recovery 74.3%.They were iden-tified as Tg-HbⅠ(homodimer)and Tg-HbⅡ(heterogeneous tetramer)by Tricine-SDS-PAGE and mass spectrome-ter.The agar diffusion method and spectrophotometric method were used to detect their antibacterial activity and peroxidase activity,respectively.Results showed that both hemoglobins exhibited antibacterial activities against Es-chetichia coli and Pseudomonas putida .The MIC values of Tg-HbⅡ towards E.coli and P .putida were 0.063 mg/mL and 0.13 mg/mL,while corresponding data of Tg-HbⅠwere 0.048 mg/mL.Both hemoglobins showed peroxidase activities,which could catalyze various of phenolic substances,such as guaiacol,pyrocatechol,hydroqui-none,phenol,L-DOPA,etc.Both hemoglobins lost their antibacterial activities against P .putida in the presence of GSH,indicating that they played the antibacterial abilities through their peroxidase activities.