CAJ | 학술논문

本实验分别针对3株低温藻株：微拟球藻 Nannochloropsis sp．ZL-12、四爿藻 Tetraselmis chui ZL-33和小球藻 Chlorella sp．ZL-45，3株中温藻株：球等鞭金藻 Isochrysis galbana CCMM5001、等鞭金藻 Isochrysis sp．CCMM5002和微拟球藻 Nannochloropsis sp．CCMM7001，3株高温藻株：微拟球藻Nannochloropsis sp．JN1、绿色巴夫藻 Pavlova viridis JN2和海洋小球藻 Chlorella sp．JN3，研究了在通入0．03％（空气）、5％、10％3个 CO2浓度梯度条件下的生长特性，同时考察了其总酯及中性脂的累积情况。结果显示，富碳培养有利于不同温度条件下9株藻株的生长，除微拟球藻 Nannochloropsis sp．CCMM7001最适生长的 CO2浓度为5％外，其余8株藻株最适生长的 CO2浓度均为10％。在低温和高温条件下，6株海洋富油微藻在通入10％ CO2时具有最大生物量产率，在中温条件下球等鞭金藻和等鞭金藻在通入10％ CO2时获得最大生物量产率，而微拟球藻在通入5％时获得最大生物量产率，随着 CO2浓度的增加，9株藻株的总脂含量和中性脂含量有明显提高。低温和中温藻株的总脂含量高于高温藻株的总脂含量，从中性脂的累积规律来看，9株藻株均在平台期的累积达到最大值，GC－MS 分析结果表明，9株微藻适合制备生物柴油的 C14～C18系脂肪酸相对含量在不同 CO2条件下基本保持不变，维持在90％左右。实验结果显示，所研究的藻株作为富油高固碳优良藻株，具备用于海洋生物质能耦合 CO2减排开发的潜力。
본실험분별침대3주저온조주：미의구조 Nannochloropsis sp．ZL-12、사장조 Tetraselmis chui ZL-33화소구조 Chlorella sp．ZL-45，3주중온조주：구등편금조 Isochrysis galbana CCMM5001、등편금조 Isochrysis sp．CCMM5002화미의구조 Nannochloropsis sp．CCMM7001，3주고온조주：미의구조Nannochloropsis sp．JN1、록색파부조 Pavlova viridis JN2화해양소구조 Chlorella sp．JN3，연구료재통입0．03％（공기）、5％、10％3개 CO2농도제도조건하적생장특성，동시고찰료기총지급중성지적루적정황。결과현시，부탄배양유리우불동온도조건하9주조주적생장，제미의구조 Nannochloropsis sp．CCMM7001최괄생장적 CO2농도위5％외，기여8주조주최괄생장적 CO2농도균위10％。재저온화고온조건하，6주해양부유미조재통입10％ CO2시구유최대생물양산솔，재중온조건하구등편금조화등편금조재통입10％ CO2시획득최대생물양산솔，이미의구조재통입5％시획득최대생물양산솔，수착 CO2농도적증가，9주조주적총지함량화중성지함량유명현제고。저온화중온조주적총지함량고우고온조주적총지함량，종중성지적루적규률래간，9주조주균재평태기적루적체도최대치，GC－MS 분석결과표명，9주미조괄합제비생물시유적 C14～C18계지방산상대함량재불동 CO2조건하기본보지불변，유지재90％좌우。실험결과현시，소연구적조주작위부유고고탄우량조주，구비용우해양생물질능우합 CO2감배개발적잠력。
Nine marine microalgae were cultured under different CO2 concentrations of ambient air (0.03%),5%and 10%,respectively.Nine marine microalgae include 3 strains of cold resisting marine microalgae (Nannochlo-ropsis sp.ZL-12,Tetraselmis chui ZL-33,Chlorella sp.ZL-45),3 strains of mesophilic marine microalgae (Isoch-rysis galbana CCMM5001,Isochrysis sp.CCMM5002 and Nannochloropsis sp.CCMM7001)and 3 strains of heat resisting marine microalgae (Nannochloropsis sp.JN1,Pavlova viridis JN2 and Chlorella sp.JN3).The growth characterization,accumulation of total and neutral lipid of these microalgae were investigated.The results showed that CO2 enrichment cultivation could increase the growth of all nine microalgae,but the optimum CO2 concentra-tions were different.The optimum CO2 concentration of Nannochloropsis sp.CCMM7001 was 5%,The optimum CO2 concentration of 8 strains of marine microalgae was 10%.3 strains of cold resisting marine microalgae and 3 strains of heat resisting marine microalgae reached the maximum biomass yield when culturing with 10% CO2 .2 strains of mesophilic marine microalgae (Isochrysis galbana CCMM5001 and Isochrysis sp.CCMM5002)run up to the maximum biomass yield when culturing with 10% CO2 .However,the maximum biomass yield of Nannochlo-ropsis sp.CCMM7001 was (122.25±1.17)mg/(L·d)when culturing with 5% CO2 .With the increased CO2 concentration,the total lipid and neutral lipid of three microalgae improved significantly.The total lipid content of 3 strains of cold resisting marine microalgae and 3 strains of mesophilic marine microalgae was higher than 3 heat resisting marine microalgae.The maximum neutral lipid content of 9 strains of microalgae could be accumulated in stationary phase.The fatty acid analysis of 9 strains of microalgae showed the relative content of C14-C18 fatty acid which suitable for biodiesel preparation maintained at 90% when culturing with different CO2 concentration. The results indicate our marine oleaginous microalgae with high carbon dioxide fixation ability are the potential ex-cellent strains for marine bioenergy development coupled with CO2 emission reduction.