CAJ | 학술논문

目的：探讨大黄素对脓毒症急性肺损伤大鼠水通道蛋白1(AQP-1)表达的影响。方法成年雄性 SD大鼠120只,体质量200~230 g。大鼠分为6组：空白对照组、假手术组、模型组、大黄素低剂量预处理组、大黄素中剂量预处理组、大黄素高剂量预处理组,每组各20只。采用盲肠结扎穿孔法复制脓毒症大鼠模型,假手术组探查取出盲肠后还纳腹腔。各组按取材时间不同又分为造模后3、6、12、24小时4个时相点,每个时相点5只大鼠。分别用实时荧光多聚酶链反应(PCR)和蛋白免疫电泳(western-blot)法检测肺组织水通道蛋白1信使 RNA(AQP-1 mRNA)和蛋白的表达。结果各组3小时AQP-1表达差异无统计学意义,造模后6小时AQP-1逐渐降低,12小时达最低。大黄素中、高剂量预处理组12小时AQP-1均显著高于同剂量其他时间点(P<0.01),且两组之间差异无统计学意义(P>0.05)。结论脓毒症可造成大鼠 AQP-1表达明显降低,早期预防性使用中高剂量大黄素可明显提高 AQP-1的表达,从而有效减轻脓毒症大鼠肺水肿的程度。
목적：탐토대황소대농독증급성폐손상대서수통도단백1(AQP-1)표체적영향。방법성년웅성 SD대서120지,체질량200~230 g。대서분위6조：공백대조조、가수술조、모형조、대황소저제량예처리조、대황소중제량예처리조、대황소고제량예처리조,매조각20지。채용맹장결찰천공법복제농독증대서모형,가수술조탐사취출맹장후환납복강。각조안취재시간불동우분위조모후3、6、12、24소시4개시상점,매개시상점5지대서。분별용실시형광다취매련반응(PCR)화단백면역전영(western-blot)법검측폐조직수통도단백1신사 RNA(AQP-1 mRNA)화단백적표체。결과각조3소시AQP-1표체차이무통계학의의,조모후6소시AQP-1축점강저,12소시체최저。대황소중、고제량예처리조12소시AQP-1균현저고우동제량기타시간점(P<0.01),차량조지간차이무통계학의의(P>0.05)。결론농독증가조성대서 AQP-1표체명현강저,조기예방성사용중고제량대황소가명현제고 AQP-1적표체,종이유효감경농독증대서폐수종적정도。
Objective To investigate the effects of emodin on aquaporin 1 (AQP-1 )expression in acute lung injury in sepsis rats.Methods A total of 120 adult SD rats weighing 200-230 g were randomly divided into six groups, blank control group,sham group,model group,low dose emodin pretreatment group,middle dose emodin pretreatment group,high dose emodin pretreatment group,20 rats in each group.The rats with sepsis were induced by cecal ligation and puncture(CLP).The abdominal cavity in sham group was closed after cecal exploration operation.Each group was further divided into four time points:3 hours,6 hours,12 hours and 24 hours after the modeling,with 5 rats in each time point,according to the specimen collection time.Rats were sacrificed at different time points to measure the expression of AQP-1 mRNA by real-time fluorescence quantitative PCR and AQP-1 protein by western-blot.Results AQP-1 expression at 3 h among different groups showed no significant difference.After modeling,AQP-1 at 6 h decreased gradually and reached the minimum 1 2 h.AQP-1 of the middle and high dose emodin pretreatment groups at 12 h were significantly higher than those of the same dose of other time points(P <0.01).There was no significant difference between the two groups(P >0.05).Conclusion Sepsis caused the AQP-1 expression of rats significantly lower.Early prophylactic use of middle or high dose emodin can significantly increase AQP-1 expression.