CAJ | 학술논문

目的：探讨氧化应激在肌萎缩侧索硬化(ALS)发病中的作用及其与病情评价指标的相关性。方法应用酶联免疫吸附测定(ELISA)法、硫代巴比妥酸法以及黄嘌呤氧化酶法分别测定39例 ALS 患者和43例正常对照者血清中8-羟基脱氧鸟苷酸(8-OhdG)、丙二醛(MDA)、谷胱甘肽(GSH)的水平；采用量表评价病情严重程度。结果ALS 组血清中8-OhdG(195.02±51.47)ng/L 和 MDA(8.34±1.67)nmol/L 明显高于对照组(122.47±56.87)ng/L和(3.47±1.17)nmol/L(P <0.05)；ALS 组血清中 GSH(18.34±2.55)nmol/L 明显低于对照组血清中 GSH(28.34±3.87)nmol/L(P <0.05)。ALS 组血清中8-OhdG、MDA 及 GSH 的水平与病情严重程度有相关性(P <0.05)。结论 ALS 患者出现了明显的氧化损伤；血清中8-OhdG、MDA 及 GSH 的水平与 ALS 病情轻重相关。
목적：탐토양화응격재기위축측색경화(ALS)발병중적작용급기여병정평개지표적상관성。방법응용매련면역흡부측정(ELISA)법、류대파비타산법이급황표령양화매법분별측정39례 ALS 환자화43례정상대조자혈청중8-간기탈양조감산(8-OhdG)、병이철(MDA)、곡광감태(GSH)적수평；채용량표평개병정엄중정도。결과ALS 조혈청중8-OhdG(195.02±51.47)ng/L 화 MDA(8.34±1.67)nmol/L 명현고우대조조(122.47±56.87)ng/L화(3.47±1.17)nmol/L(P <0.05)；ALS 조혈청중 GSH(18.34±2.55)nmol/L 명현저우대조조혈청중 GSH(28.34±3.87)nmol/L(P <0.05)。ALS 조혈청중8-OhdG、MDA 급 GSH 적수평여병정엄중정도유상관성(P <0.05)。결론 ALS 환자출현료명현적양화손상；혈청중8-OhdG、MDA 급 GSH 적수평여 ALS 병정경중상관。
Objective To explore the effects of oxidative stress in amyotrophic lateral sclerosis (ALS)and its relevance with the disease evaluation index.Methods The level of 8-hydroxy deoxyguansine acid (8-OhdG ), malondialdehyde(MDA)and glutathione(GSH)in 39 cases of ALS and 43 normal controls were detected by enzyme-linked immunosorbent assay(ELISA),thiobarbituric acid and xanthine oxidase technique.The severity of disease was evaluated by using rating scale.Results The 8-OhdG (1 95.02±5 1.47)ng/L and MDA (8.34±1.67)nmol/L in the serum of patients were significantly higher than those in the control group (122.47 ±56.87)ng/L and (3.47±1.17) nmol/L(P <0.05).The GSH level (18.34±2.55)nmol/L in the serum of patients was significantly lower than that in the control group(28.34±3.87)nmol/L(P <0.01).②There were relevance between the level of 8-OhdG,MDA and GSH and the disease severity in the patients (P <0.05 ).Conclusion Obvious oxidative damage exists in ALS patients.The levels of 8-OhdG,MAD and GSH are closely related to the severity of ALS patients.