CAJ | 학술논문

目的：探讨九项呼吸道病原联合检测在诊断小儿急性下呼吸道感染非细菌病原学中的临床应用价值，并了解小儿急性下呼吸道感染的病原学特点。方法应用九项呼吸道联合检测试剂(间接免疫荧光法)，检测小儿急性下呼吸道感染1389例血清标本的九项病原体 IgM 抗体，包括嗜肺军团菌、肺炎支原体、Q 热立克次体、肺炎衣原体、腺病毒、呼吸道合胞病毒、甲型流感病毒、乙型流感病毒、副流感病毒1、2和3型。结果总感染率为42.6%(592/1389)，其中以肺炎支原体最为多见，其次为乙型流感病毒；混合感染率达14.5%(201/1389)，以支原体感染与乙型流感病毒混合感染最多。但1岁以下组阳性率较低。结论间接免疫荧光法九项呼吸道病原体联合检测是检测小儿急性下呼吸道感染病原体的一种具有高灵敏度和高特异性且操作简便的方法，适合1岁以上小儿的临床应用。
목적：탐토구항호흡도병원연합검측재진단소인급성하호흡도감염비세균병원학중적림상응용개치，병료해소인급성하호흡도감염적병원학특점。방법응용구항호흡도연합검측시제(간접면역형광법)，검측소인급성하호흡도감염1389례혈청표본적구항병원체 IgM 항체，포괄기폐군단균、폐염지원체、Q 열립극차체、폐염의원체、선병독、호흡도합포병독、갑형류감병독、을형류감병독、부류감병독1、2화3형。결과총감염솔위42.6%(592/1389)，기중이폐염지원체최위다견，기차위을형류감병독；혼합감염솔체14.5%(201/1389)，이지원체감염여을형류감병독혼합감염최다。단1세이하조양성솔교저。결론간접면역형광법구항호흡도병원체연합검측시검측소인급성하호흡도감염병원체적일충구유고령민도화고특이성차조작간편적방법，괄합1세이상소인적림상응용。
Objective To study the diagnosis of 9 assays for nonbacterial pathogens in acute lower respiratory infection(ALRI)among children,and to investigate the pathogenic characteristics.Methods Nine-item combined assays were used to detect IgM antibody of nine pathogens in serum specimens of 1 389 children with acute lower respiratory tract infection,including Legionella pneumophila,Mycoplasma pneumophila,Coxiella burneti,Chlamydia pneumophila,adenovirus,respiratory syncytial virus(RSV),influenza A virus,influenza B virus and parainfluenza virus types 1,2 and 3,via indirect immunofluorescence assay.Results The total infection rate was 42.6%(592/1 389),and Mycoplasma pneumoniae was the most common pathogen,followed by influenza B virus;mixed infection rate was 14.5%(201/1 389),in which the mixed infection of Mycoplasma and influenza B virus was most common.However, the positive rate of patients under the age of 1 year old was lower.Conclusion Nine-item combined assay is a method with the features as high sensitivity,high specificity and easy doing in detecting pathogens of children with acute lower respiratory tract infection,and it is suitable for clinical application in more than 1-year-old children.