CAJ | 학술논문

蒲公英中内含的黄酮类物质具有较高药用价值，用双水相萃取法提取植物中有效成分是新型提取高附加值生物质的有效方法。本文考察了PEG/(NH4)2SO4双水相体系萃取分离蒲公英总黄酮时聚乙二醇相对分子质量、PEG质量分数、(NH4)2SO4质量分数、温度、pH值5个因素对分配行为的影响，并通过正交实验优化了工艺条件。结果表明最佳双水相提取工艺条件为：(NH4)2SO4质量分数18%，PEG1000质量分数23%，pH值5.34，提取温度25℃，NaCl盐的存在与否对萃取影响很小，蒲公英中总黄酮的提取率可达5.47%。因此，使用双水相法提取蒲公英总黄酮是该类提取技术中一种更加绿色环保和高效的方法。
포공영중내함적황동류물질구유교고약용개치，용쌍수상췌취법제취식물중유효성분시신형제취고부가치생물질적유효방법。본문고찰료PEG/(NH4)2SO4쌍수상체계췌취분리포공영총황동시취을이순상대분자질량、PEG질량분수、(NH4)2SO4질량분수、온도、pH치5개인소대분배행위적영향，병통과정교실험우화료공예조건。결과표명최가쌍수상제취공예조건위：(NH4)2SO4질량분수18%，PEG1000질량분수23%，pH치5.34，제취온도25℃，NaCl염적존재여부대췌취영향흔소，포공영중총황동적제취솔가체5.47%。인차，사용쌍수상법제취포공영총황동시해류제취기술중일충경가록색배보화고효적방법。
Total flavonoids in Taraxacum mongolicum are a highly effective medicine,and aqueous two-phase extraction process is an effective technique for extracting biomaterials from plant. Effect of molecular weight and mass fraction of PEG,mass fraction of (NH4)2SO4,temperature and pH of aqueous two-phase system were investigated during extraction of total flavonoids from Taraxacum mongolicum. The optimal extraction conditions were obtained through orthogonal experiments. The optimal condition thus obtained is as follows:the average molecular weight of PEG at 1000,the mass fraction of PEG1000 at 23%,the mass fraction of (NH4)2SO4 at 18%,temperature at 25℃,pH at 5.34. The NaCl addition has little effect on flavonoids partition. The extraction yield of total flavonoids from Taraxacum mongolicum can reach up to 5.47% under this optimal condition. Compared with other extraction methods,the aqueous two-phase extraction process is a more environment-friendly and highly efficient technique.