CAJ | 학술논문

目的：检测 E2F1和 ki-67蛋白在乳腺浸润性导管癌（invasive ductal carcinoma，IDC）中的表达，为乳腺癌的发生发展及预后判断提供一定的依据。方法：采用免疫组化 SP 法检测91例 IDC、18例不典型增生组织、30例正常乳腺组织中 E2F1、ki-67蛋白表达情况，分析二者与临床病理因素之间的关系。结果：① E2F1和 ki-67蛋白表达率在 IDC 组织分别为47.3%（43/91）和69.2%（63/91），在不典型增生组分别为11.1%（2/18）和16.7%（3/18），正常组均不表达，各组之间相比较，差异显著（<0.05）。②乳腺癌组织 E2F1和 ki-67阳性表达与腋窝淋巴结转移、肿瘤大小、组织学分级、TNM 分期有关（<0.05），与患者年龄无关（>0.05）；E2F1和 ki-67在乳腺癌中的阳性表达呈显著正相关（<0.05）。结论：E2F1、ki-67蛋白与乳腺癌的发生发展有关，提示它们可能是参与IDC 病程进展的重要因子，二者联合检测对患者预后评估具有重要价值。
목적：검측 E2F1화 ki-67단백재유선침윤성도관암（invasive ductal carcinoma，IDC）중적표체，위유선암적발생발전급예후판단제공일정적의거。방법：채용면역조화 SP 법검측91례 IDC、18례불전형증생조직、30례정상유선조직중 E2F1、ki-67단백표체정황，분석이자여림상병리인소지간적관계。결과：① E2F1화 ki-67단백표체솔재 IDC 조직분별위47.3%（43/91）화69.2%（63/91），재불전형증생조분별위11.1%（2/18）화16.7%（3/18），정상조균불표체，각조지간상비교，차이현저（<0.05）。②유선암조직 E2F1화 ki-67양성표체여액와림파결전이、종류대소、조직학분급、TNM 분기유관（<0.05），여환자년령무관（>0.05）；E2F1화 ki-67재유선암중적양성표체정현저정상관（<0.05）。결론：E2F1、ki-67단백여유선암적발생발전유관，제시타문가능시삼여IDC 병정진전적중요인자，이자연합검측대환자예후평고구유중요개치。
Objectiye:To evaluate the expression of E2F1 and ki-67 protein in IDC,and to provide a theoretical basis for the development and prognosis of IDC. Methods:The SP methods was used to detect the expression of E2F1 and ki-67protein in 91 cases of IDC,18 cases of atypical hyperplasia and 30 cases of normal breast tissues of non-cancerous breast disease,and its relationships with clinicopathological features were analyzed. Result:①The positive expression rate of E2F1 and ki-67protein was 47. 3% and 69. 2% in IDC,11. 1% and 16. 7% in atypical hyperplasia,respectively. The expression of E2F1 and ki-67 protein was significantly different among three groups( < 0. 05). ② The expression of E2F1 and ki-67 was positively correlated with lymph node status,tumor size,histological grading and TNM classification of tumor( < 0. 05),but not correlated with age. The expression of E2F1 and ki-67was positively correlated in IDC( =0. 297, < 0. 05). Conclusion:E2F1 and ki-67may be involved the occurrence and progress in breast cancer. The expres-sion of E2F1 and ki-67 are correlated with the prognosis of patients. Combined detection of E2F1 and ki-67 may be more helpful to evaluated the prognosis of breast cancer.