CAJ | 학술논문

基因芯片又称为DNA微阵列，是指将大量核酸片段以预先设计的方式固定在载体上组成密集分子阵列，与荧光素或其他方式标记的样品进行杂交，通过检测杂交信号的强弱来判断样品中有无靶分子以及对靶分子进行定量，是一种研究生物大分子功能的新技术。在衣原体研究方面，基因芯片主要应用于衣原体的检测与分型、感染机制的研究、特定基因作用分析、毒力及耐药基因的筛选等。
기인심편우칭위DNA미진렬，시지장대량핵산편단이예선설계적방식고정재재체상조성밀집분자진렬，여형광소혹기타방식표기적양품진행잡교，통과검측잡교신호적강약래판단양품중유무파분자이급대파분자진행정량，시일충연구생물대분자공능적신기술。재의원체연구방면，기인심편주요응용우의원체적검측여분형、감염궤제적연구、특정기인작용분석、독력급내약기인적사선등。
Gene chip is also known as DNA microarray , it is a new technology to study the function of biomacromolecule . The principle of microarray is to fix a large number of nucleotide sequences which are designed in advance on the carrier to compose of dense molecular array , hybridized samples labeled with fluorescein or other ways .Finally, it is in detection of hybridization signal to measure and quantitation target molecules in the sample .In this paper , it is reviewed microarray ap-plication in Chlamydia research including detection and typing , infection mechanism , analysis in specific genes function , and screening virulence and resistance gene .