CAJ | 학술논문

【目的】检测微小 RNA（microRNA，miRNA）-613在甲状腺乳头状癌、结节性甲状腺肿和癌旁正常甲状腺组织中的表达差异，探讨miRNA-613在甲状腺乳头状癌中表达的意义。【方法】收集自2012年3月至2013年9月于本院甲状腺外科行甲状腺手术病人甲状腺乳头状癌组织及癌旁远端非瘤正常甲状腺组织44例，结节性甲状腺肿组织31例。采用 Real-time PCR的方法定量分析 miRNA-613在甲状腺乳头状癌组织、癌旁正常组织及结节性甲状腺组织中的表达情况，并分析其与甲状腺乳头状癌临床病理特征的相关性。【结果】miRNA-613在甲状腺乳头状癌中表达量明显低于非肿瘤组织（P＜0．05），非肿瘤组织间比较差异无统计学意义（P＞0．05）。miRNA-613的表达量与甲状腺乳头状癌有无腺外浸润明显相关（P ＜0．05）。【结论】在甲状腺乳头状癌组织中，miRNA-613的表达降低，且与甲状腺乳头状癌的侵袭性相关。
【목적】검측미소 RNA（microRNA，miRNA）-613재갑상선유두상암、결절성갑상선종화암방정상갑상선조직중적표체차이，탐토miRNA-613재갑상선유두상암중표체적의의。【방법】수집자2012년3월지2013년9월우본원갑상선외과행갑상선수술병인갑상선유두상암조직급암방원단비류정상갑상선조직44례，결절성갑상선종조직31례。채용 Real-time PCR적방법정량분석 miRNA-613재갑상선유두상암조직、암방정상조직급결절성갑상선조직중적표체정황，병분석기여갑상선유두상암림상병리특정적상관성。【결과】miRNA-613재갑상선유두상암중표체량명현저우비종류조직（P＜0．05），비종류조직간비교차이무통계학의의（P＞0．05）。miRNA-613적표체량여갑상선유두상암유무선외침윤명현상관（P ＜0．05）。【결론】재갑상선유두상암조직중，miRNA-613적표체강저，차여갑상선유두상암적침습성상관。
[Objective]To detect the difference of the expression of microRNA-613(miR-613)in papillary thyroid carcinoma(PTC),nodular goiter and normal thyroid tissues surrounding the cancer,and to explore the significance of miR-613 in PTC.[Methods]A total of 190 cases of PTC undergoing thyroid resection,44 cases of distal non-tumor normal thyroid tissue surrounding the cancer and 3 1 cases of nodular goiter in our hospital from March 2012 to Sept.2013 were collected.The expression level of miR-613 in PTC tissue,normal thyroid tissue surrounding the cancer and nodular goiter tissue were assayed by real-time polymerase chain reaction (PCR).The correlation between miR-6 1 3 expression and clinicopathologic features of PTC was analyzed.[Re-sults]The expression level of miR-6 1 3 in PTC tissue was obviously lower than that in non-tumor tissue(P<0.05),but there was no significant difference among non-tumor tissues(P>0.05).The expression level of miR-613 was correlated with extra-thyroid invasion of PTC(P<0.05).[Conclusion]The expression level of miR-6 1 3 in PTC is decreased and correlated with tumor invasiveness.