CAJ | 학술논문

【目的】探讨小鼠嗜酸粒细胞性支气管炎模型（EB模型）与哮喘模型的蛋白组学差异表达。【方法】EB模型小鼠（实验组）、哮喘模型小鼠（哮喘组）及对照组小鼠各4只，取肺组织提取总蛋白质，采用基质辅助电离解析飞行时间质谱对蛋白质进行序列分析，并比较三组肺组织蛋白差异。【结果】双向电泳图像显示三组肺组织蛋白表达比较差异有显著性（P＜0．05），经质谱检测肽质量指纹谱与标准分子量、等电点对照分析鉴定出20个蛋白，分别为谷胱甘肽-S-转移酶 M1（GSTM1）、热休克蛋白 B1（HSPB1）等，其中 GSTM1在哮喘组表达下调、HSPB1在哮喘组表达上调。【结论】GSTM1、HSPB1等可能参与气道高反应性的发生机制。
【목적】탐토소서기산립세포성지기관염모형（EB모형）여효천모형적단백조학차이표체。【방법】EB모형소서（실험조）、효천모형소서（효천조）급대조조소서각4지，취폐조직제취총단백질，채용기질보조전리해석비행시간질보대단백질진행서렬분석，병비교삼조폐조직단백차이。【결과】쌍향전영도상현시삼조폐조직단백표체비교차이유현저성（P＜0．05），경질보검측태질량지문보여표준분자량、등전점대조분석감정출20개단백，분별위곡광감태-S-전이매 M1（GSTM1）、열휴극단백 B1（HSPB1）등，기중 GSTM1재효천조표체하조、HSPB1재효천조표체상조。【결론】GSTM1、HSPB1등가능삼여기도고반응성적발생궤제。
[Obj ective]To explore the differential expression of proteomics of eosinophilic bronchitis mouse model and asthma mouse model.[Methods]Four rats in each group including eosinophilic bronchitis model(EB model)(experimental group),asthma model(asthma group)and control group.Total protein was extracted from lung tissue.Matrix-assisted laser desorption/ionization time of flight mass spectrometry was used for sequence a-nalysis of proteins.[Results]Two dimensional electrophoresis image showed that there was difference in the ex-pression of protein among 3 groups(P<0.05).Twenty proteins such as glutathione-S-transferase M1(GSTM1) and heat shock protein B1(HSPB1)were identified through the analysis of peptide mass fingerprint spectrum of mass spectrometry contrasted with standard molecular weight and isoelectric point.GSTM1 was down-regulated in asthma group,while HSPB1 was up-regulated in asthma group.[Conclusion]GSTM1 and HSPB1 may involve in the occurrence of airway hyperresponsiveness.