CAJ | 학술논문

目的：探讨野木瓜注射液对炎性疼痛模型小鼠的消炎镇痛作用及机制。方法：采用足底皮内注射角叉菜胶制备小鼠炎性疼痛模型，对治疗组小鼠皮下注射12．5％、50％和100％野木瓜注射液，于4、12、24、48 h后测各组小鼠足趾肿胀值和足底热痛阈值，并取小鼠第五腰椎（L5）背根神经节（DRG）行免疫组化染色，检测环氧化酶2（COX-2）蛋白的表达。结果：造模后，模型组小鼠足趾肿胀持续明显，足底热痛阈值持续降低。至48 h时，与对照组相比，模型组足趾肿胀明显；与对照组相比，模型组足底热痛阈值显著降低。与模型组相比，低、中、高剂量野木瓜注射液均可降低小鼠足趾肿胀度，恢复小鼠的热痛阈值，且下调L5 DRG中COX-2的蛋白表达至模型组的70％、66％和30％。结论：野木瓜注射液能降低伤害性刺激诱导的L5 DRG中COX-2蛋白表达，起到抗炎镇痛的效果。
목적：탐토야목과주사액대염성동통모형소서적소염진통작용급궤제。방법：채용족저피내주사각차채효제비소서염성동통모형，대치료조소서피하주사12．5％、50％화100％야목과주사액，우4、12、24、48 h후측각조소서족지종창치화족저열통역치，병취소서제오요추（L5）배근신경절（DRG）행면역조화염색，검측배양화매2（COX-2）단백적표체。결과：조모후，모형조소서족지종창지속명현，족저열통역치지속강저。지48 h시，여대조조상비，모형조족지종창명현；여대조조상비，모형조족저열통역치현저강저。여모형조상비，저、중、고제량야목과주사액균가강저소서족지종창도，회복소서적열통역치，차하조L5 DRG중COX-2적단백표체지모형조적70％、66％화30％。결론：야목과주사액능강저상해성자격유도적L5 DRG중COX-2단백표체，기도항염진통적효과。
AIM: To investigate the effect of injection stauntoniae ( IS) on inflammatory pain responses in mice.METHODS:The carrageenan test was used to determine the anti-inflammatory and analgesic effects of IS .Except for control group, the mice in other groups received an injection of λ-carrageenan solution (1%, 50 μL) into the plantar region of the left hind paws , followed by a subcutaneous injection of IS at doses of 12.5%, 50%and 100%or equal vol-ume of 0.9%NaCl.Both paw edema and hyperalgesia to thermal stimulation were measured 4 h, 12 h, 24 h and 48 h after the injection of λ-carrageenan solution.The lumbar-5 (L5) dorsal root ganglions (DRGs) of the mice were taken to inves-tigate the cyclooxygenase 2 ( COX-2) expression by immunohistochemical staining .RESULTS:Subcutaneous injection of IS potently inhibited paw edema and hyperalgesia induced by λ-carrageenan in the mice accompanied with the inhibition of COX-2 protein expression in L 5 DRGs.CONCLUSION:IS exerts the anti-inflammatory and analgesic effects on the in-flammatory responses by inhibiting the protein expression of COX-2 in DRGs.