中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
7期
1184-1191
,共8页
夏忠胜%钟娃%于涛%练国达%周慧敏%陈广成
夏忠勝%鐘娃%于濤%練國達%週慧敏%陳廣成
하충성%종왜%우도%련국체%주혜민%진엄성
结肠肿瘤%侧群细胞%微小RNA%miR-5000-3p%miR-5009-3p%miR-552
結腸腫瘤%側群細胞%微小RNA%miR-5000-3p%miR-5009-3p%miR-552
결장종류%측군세포%미소RNA%miR-5000-3p%miR-5009-3p%miR-552
Colonic neoplasms%Side population cells%MicroRNA%miR-5000-3p%miR-5009-3p%miR-552
目的：探讨结肠癌侧群（ SP）细胞在结肠癌多药耐药性中的作用及其microRNA生物标志。方法：结肠癌SP细胞的分选采用流式细胞术，细胞活力的测定采用MTT法，microRNA表达谱的检测采用microRNA芯片，microRNA表达的验证采用实时荧光定量PCR。结果：（1）HCT-15、HT-29及LoVo结肠癌细胞系中SP细胞的比例分别为16．75％、13．02％及9．52％。（2）化疗药（5-氟尿嘧啶、草酸铂及阿霉素）对3种结肠癌细胞系SP细胞的IC50均明显高于对非SP细胞的IC50（P＜0．05）。（3）microRNA芯片检测表明miR-5000-3p、miR-5009-3p及miR-552在3种结肠癌细胞系的SP细胞中表达均上调，实时荧光定量PCR亦证实此结果。结论： miR-5000-3p、miR-5009-3p及miR-552在结肠癌细胞系的SP细胞中表达上调，有可能成为结肠癌SP细胞的潜在microRNA生物标志。
目的：探討結腸癌側群（ SP）細胞在結腸癌多藥耐藥性中的作用及其microRNA生物標誌。方法：結腸癌SP細胞的分選採用流式細胞術，細胞活力的測定採用MTT法，microRNA錶達譜的檢測採用microRNA芯片，microRNA錶達的驗證採用實時熒光定量PCR。結果：（1）HCT-15、HT-29及LoVo結腸癌細胞繫中SP細胞的比例分彆為16．75％、13．02％及9．52％。（2）化療藥（5-氟尿嘧啶、草痠鉑及阿黴素）對3種結腸癌細胞繫SP細胞的IC50均明顯高于對非SP細胞的IC50（P＜0．05）。（3）microRNA芯片檢測錶明miR-5000-3p、miR-5009-3p及miR-552在3種結腸癌細胞繫的SP細胞中錶達均上調，實時熒光定量PCR亦證實此結果。結論： miR-5000-3p、miR-5009-3p及miR-552在結腸癌細胞繫的SP細胞中錶達上調，有可能成為結腸癌SP細胞的潛在microRNA生物標誌。
목적：탐토결장암측군（ SP）세포재결장암다약내약성중적작용급기microRNA생물표지。방법：결장암SP세포적분선채용류식세포술，세포활력적측정채용MTT법，microRNA표체보적검측채용microRNA심편，microRNA표체적험증채용실시형광정량PCR。결과：（1）HCT-15、HT-29급LoVo결장암세포계중SP세포적비례분별위16．75％、13．02％급9．52％。（2）화료약（5-불뇨밀정、초산박급아매소）대3충결장암세포계SP세포적IC50균명현고우대비SP세포적IC50（P＜0．05）。（3）microRNA심편검측표명miR-5000-3p、miR-5009-3p급miR-552재3충결장암세포계적SP세포중표체균상조，실시형광정량PCR역증실차결과。결론： miR-5000-3p、miR-5009-3p급miR-552재결장암세포계적SP세포중표체상조，유가능성위결장암SP세포적잠재microRNA생물표지。
AIM:To investigate the role of side population ( SP) cells in multidrug resistance of colon cancer cells and microRNA biomarkers of SP cells in colon cancer cells .METHODS:SP cells in colon cancer cells were sorted by flow cytometry .The cell viability was measured by MTT method .MicroRNA expression profiles were detected by mi-croRNA chip.MicroRNA expression was verified by real-time PCR.RESULTS:The ratios of SP cells in HCT-15, HT-29 and LoVo colon cancer cell lines were 16.75%, 13.02%and 9.52%, respectively.In all 3 colon cancer cell lines, IC50 of the antitumor drugs including 5-fluorouracil , oxaliplatin and adriamycin for the SP cells were significantly higher than those for non-SP cells (P<0.05).MicroRNA profiling showed that miR-5000-3p, miR-5009-3p and miR-552 were all up-regulated in the SP cells of all 3 colon cancer cell lines .This result was verified by real-time PCR.CONCLUSION:miR-5000-3p, miR-5009-3p and miR-552 are all up-regulated in the SP cells of colon cancer cell lines , and may be the poten-tial microRNA biomarkers of SP cells in colon cancer .
