CAJ | 학술논문

目的：研究乙醇对低分化鼻咽癌CNE-2Z细胞氯通道的影响并探讨其分子机制。方法：MTT检测乙醇对细胞生长的影响；全细胞膜片钳技术记录乙醇对氯电流的影响；应用氯通道阻断剂分析该电流的特性；siR-NA干扰技术分析乙醇敏感氯通道的分子本质。结果：在等渗灌流液中记录到微弱的背景氯电流，随灌流液中乙醇浓度（0．17～170 mmol/L）的升高，所激活的氯电流呈倒U型曲线状，17 mmol/L的乙醇激活电流达到峰值，电流呈较明显的外向优势，能被高渗液及氯通道阻断剂5-nitro-2-（3-phenylpropylamino）benzoic acid（NPPB）所阻断；干扰ClC-3氯通道基因表达后乙醇激活的氯电流明显减小。结论：乙醇可能通过激活CNE-2Z细胞的ClC-3氯通道，诱导氯电流的产生。
목적：연구을순대저분화비인암CNE-2Z세포록통도적영향병탐토기분자궤제。방법：MTT검측을순대세포생장적영향；전세포막편겸기술기록을순대록전류적영향；응용록통도조단제분석해전류적특성；siR-NA간우기술분석을순민감록통도적분자본질。결과：재등삼관류액중기록도미약적배경록전류，수관류액중을순농도（0．17～170 mmol/L）적승고，소격활적록전류정도U형곡선상，17 mmol/L적을순격활전류체도봉치，전류정교명현적외향우세，능피고삼액급록통도조단제5-nitro-2-（3-phenylpropylamino）benzoic acid（NPPB）소조단；간우ClC-3록통도기인표체후을순격활적록전류명현감소。결론：을순가능통과격활CNE-2Z세포적ClC-3록통도，유도록전류적산생。
AIM:To study the effects and mechanisms of ethanol on chloride channels in poorly differentiated nasopharyngeal carcinoma CNE-2Z cells.METHODS:The effect of ethanol on the cell growth was analyzed by MTT as-say.The technique of whole-cell patch-clamp was used to detect the chloride current .The characteristics of the chloride current were analyzed by using the chloride channel blockers .The siRNA technique was used to analyze the molecular basis of the ethanol-sensitive chloride channels .RESULTS: Under isotonic conditions , the background current was weak and stable.Ethanol at concentrations of 0.17~170 mmol/L activated a chloride current in a concentration-dependent manner (an inverted U-shape), with a maximum effect at the concentration of 17 mmol/L.The currents showed obviously outward rectification and were susceptible to extracellular hypertonicity and the chloride channel blocker , 5-nitro-2-(3-phenylpropyl-amino) benzoic acid ( NPPB) .ClC-3 siRNA obviously decreased the currents activated by ethanol .CONCLUSION:Ex-tracellular ethanol induces chloride currents through activating the ClC-3 chloride channels .