CAJ | 학술논문

目的：探讨缝隙连接蛋白43（ Cx43）侧面化在七氟醚预处理减轻离体大鼠心脏心肌缺血/再灌注性心律失常方面的作用。方法雄性Wistar 大鼠24只，随机分为3组（ n＝8）：对照组（ C组）、缺血/再灌注组（ IR组）、3％七氟醚预处理组（ SP组）。采用Langendorff灌注装置进行离体心脏灌注：C组持续灌注90 min；IR组平衡30 min，缺血30 min，再灌注30 min；SP组平衡10 min，3％七氟醚预处理15 min，洗脱5 min，缺血30 min，再灌注30 min。记录各组的心电图，并于再灌注结束后取左心室游离壁通过免疫荧光观察 Cx43的侧面化情况。结果与IR组相比，SP组的期前收缩次数明显减少（P＜0．05），室性心动过速和心室纤颤的时程明显缩短（P＜0．05），心室纤颤发生率降低（P＜0．05），心律失常评分降低（P＜0．05）。 Cx43的侧面化情况SP组明显优于IR组（P＜0．05）。结论七氟醚能有效减轻缺血/再灌注性心律失常的发生，其机制可能与改善Cx43的侧面化程度有关。
목적：탐토봉극련접단백43（ Cx43）측면화재칠불미예처리감경리체대서심장심기결혈/재관주성심률실상방면적작용。방법웅성Wistar 대서24지，수궤분위3조（ n＝8）：대조조（ C조）、결혈/재관주조（ IR조）、3％칠불미예처리조（ SP조）。채용Langendorff관주장치진행리체심장관주：C조지속관주90 min；IR조평형30 min，결혈30 min，재관주30 min；SP조평형10 min，3％칠불미예처리15 min，세탈5 min，결혈30 min，재관주30 min。기록각조적심전도，병우재관주결속후취좌심실유리벽통과면역형광관찰 Cx43적측면화정황。결과여IR조상비，SP조적기전수축차수명현감소（P＜0．05），실성심동과속화심실섬전적시정명현축단（P＜0．05），심실섬전발생솔강저（P＜0．05），심률실상평분강저（P＜0．05）。 Cx43적측면화정황SP조명현우우IR조（P＜0．05）。결론칠불미능유효감경결혈/재관주성심률실상적발생，기궤제가능여개선Cx43적측면화정도유관。
Objective To explore the role that lateralized connexin 43 takes in attenuating ischemia/reperfusion-induced arrhythmias in isolated rat hearts by sevoflurane preconditioning .Methods Twenty-four male Wistar rats were randomly divided into a control group (Group C), an ischemia/reperfusion group (Group IR), and 3%sevoflurane pre-conditioning group ( Group SP ) ( n =8 ) .Then, the Langendoff apparatus was adopted to infuse fluids into isolated hearts.Group C was perfused with K -H solution for 90 min.Group IR was first perfused with K -H solution for 30 min, followed by 30 min of ischemia before 30 min of reperfusion.Group SP was first persfused with K -H solution for 10 min, then received 15 min preconditioning by 3%sevoflurane and 5 min of wash-out, followed by 30 min of ische-mia before 30 min of reperfusion.The ECG data of each group were recorded .The level of lateralized Cx43 was measured by immunofluorescence .Results Compared with the IR group , the SP group presented less frequent premature contrac-tion and ventricular fibrillation , shorter courses of ventricular tachycardia and fibrillation , lower scores of arrhythmias , and superior levels of lateralized Cx43 (each P<0.05).Conclusion Sevoflurane preconditioning can effectively atten-uate ischemia/reperfusion-induced arrhythmias , which my attribute to an increased amount of lateralized Cx 43.