徐州医学院学报
徐州醫學院學報
서주의학원학보
ACTA ACADEMIAE MEDICINAE XUZHOU
2014年
7期
434-436
,共3页
傅薇薇%陈媛媛%赵晶%高超
傅薇薇%陳媛媛%趙晶%高超
부미미%진원원%조정%고초
特异AT序列结合蛋白1%多药耐药基因%大肠癌
特異AT序列結閤蛋白1%多藥耐藥基因%大腸癌
특이AT서렬결합단백1%다약내약기인%대장암
special AT-rich sequence-binding protein 1%multidrug resistance gene%colorectal cancer
目的：探讨特异AT序列结合蛋白1（special AT-rich sequence-binding protein 1，SATB1）基因表达对大肠癌细胞SW620内多药耐药基因（multidrug resistance gene，MDR1）表达的影响，并初步探讨SATB1基因参与大肠癌多药耐药的机制。方法将3对靶向针对SATB1基因的小片段干扰核糖核酸（ small interfering RNA ，siR-NA）与脂质体转染复合物转染至SW620细胞，采用Western blot 分别检测SATB1蛋白和P-糖蛋白（ P-glycopro-tein，P-gp）的表达，CCK8法检测基因转染前后SW620细胞对化疗药物5-氟尿嘧啶（5-fluouracil，5-Fu）、长春新碱的敏感性。结果 Western blot显示转染后SW 620细胞内SATB1蛋白表达显著降低（P＜0．05），而P-gp的表达水平亦显著降低（P＜0．05）；CCK8法检测结果显示SATB1基因转染后SW620细胞增强了对5-Fu、长春新碱的敏感性（P＜0．05）。结论下调SATB1基因可以下调SW620细胞中MDR1基因的表达，逆转SW620细胞的多药耐药。
目的：探討特異AT序列結閤蛋白1（special AT-rich sequence-binding protein 1，SATB1）基因錶達對大腸癌細胞SW620內多藥耐藥基因（multidrug resistance gene，MDR1）錶達的影響，併初步探討SATB1基因參與大腸癌多藥耐藥的機製。方法將3對靶嚮針對SATB1基因的小片段榦擾覈糖覈痠（ small interfering RNA ，siR-NA）與脂質體轉染複閤物轉染至SW620細胞，採用Western blot 分彆檢測SATB1蛋白和P-糖蛋白（ P-glycopro-tein，P-gp）的錶達，CCK8法檢測基因轉染前後SW620細胞對化療藥物5-氟尿嘧啶（5-fluouracil，5-Fu）、長春新堿的敏感性。結果 Western blot顯示轉染後SW 620細胞內SATB1蛋白錶達顯著降低（P＜0．05），而P-gp的錶達水平亦顯著降低（P＜0．05）；CCK8法檢測結果顯示SATB1基因轉染後SW620細胞增彊瞭對5-Fu、長春新堿的敏感性（P＜0．05）。結論下調SATB1基因可以下調SW620細胞中MDR1基因的錶達，逆轉SW620細胞的多藥耐藥。
목적：탐토특이AT서렬결합단백1（special AT-rich sequence-binding protein 1，SATB1）기인표체대대장암세포SW620내다약내약기인（multidrug resistance gene，MDR1）표체적영향，병초보탐토SATB1기인삼여대장암다약내약적궤제。방법장3대파향침대SATB1기인적소편단간우핵당핵산（ small interfering RNA ，siR-NA）여지질체전염복합물전염지SW620세포，채용Western blot 분별검측SATB1단백화P-당단백（ P-glycopro-tein，P-gp）적표체，CCK8법검측기인전염전후SW620세포대화료약물5-불뇨밀정（5-fluouracil，5-Fu）、장춘신감적민감성。결과 Western blot현시전염후SW 620세포내SATB1단백표체현저강저（P＜0．05），이P-gp적표체수평역현저강저（P＜0．05）；CCK8법검측결과현시SATB1기인전염후SW620세포증강료대5-Fu、장춘신감적민감성（P＜0．05）。결론하조SATB1기인가이하조SW620세포중MDR1기인적표체，역전SW620세포적다약내약。
Objective To investigate the effects of special AT -rich sequence -binding protein 1 (SATB1) gene on the expression of multidrug resistance 1 (MDR1) gene in colorectal cancer cell line SW620, and to explore the mech-anism by which SATB1 gene involves in the MDR of colorectal cancer .Methods The specific sequences of SATB 1 small interfering RNA ( siRNA) were designed and synthesized in vitro , then transfected into SW620 cells using lipo-fectamine 2000 reagent.The levels of SATB1 and P-gp were examined by Western blot .The cellular sensitivity to che-motherapeutic treatment was detected by CCK 8 method.Results According to western blot, the expression of SATB1 in SW620 was significantly decreased (P<0.05), in contrast with a reduced level of P -gp (P<0.05).Meanwhile, CCK8 method indicated that SW 620 cells enhanced their cellular sensitivity to chemotherapeutic treatment after SATB 1 gene was silenced (P<0.05).Conclusion Down -regulation of SATB1 by siRNA could reduce the expression of MDR1 gene in SW620 cells so as to reverse the MDR of the cells .
