CAJ | 학술논문

α-淀粉酶作为一种十分重要的酶制剂，目前工业上都是以微生物发酵法大规模生产的。从耐高温菌株Geobacillus. sp. POT5基因组中，用PCR的方法扩增得到该菌的α-淀粉酶基因，此基因被克隆，测序并在原核Escherichia coli表达系统中进行表达，并对该重组酶的酶学性质进行初步分析。
α-정분매작위일충십분중요적매제제，목전공업상도시이미생물발효법대규모생산적。종내고온균주Geobacillus. sp. POT5기인조중，용PCR적방법확증득도해균적α-정분매기인，차기인피극륭，측서병재원핵Escherichia coli표체계통중진행표체，병대해중조매적매학성질진행초보분석。
α-amylase is a very important enzyme,the mass production ofα-amylase is based on microorganism fermentation in the industry at present. The gene ofα-amylases(amyP)is amplified from the genome of the thermostable strain Geoba- cillus. sp. POT5 by PCR. This gene is cloned,Sequence analysis and expressed in procaryotic expression system in E.coli. This study also developes a preliminary analysis of enzyme properties about the recombinantα-amylase.