CAJ | 학술논문

从培养基添加的成分、 pH值、培养温度以及保护剂等方面，研究对丹贝冻干菌剂存活率的影响，并探究菌剂的稳定性。结果表明，当丹贝菌在PDA培养基中加入0.5%山梨醇或0.5%麦芽糖抗冻剂，培养基pH值在6.5或8.5，31℃预培养48 h时，预冻保护剂采用脱脂乳10%，甘油10%，山梨醇15%时，冻干后菌种的存活率最高，可达73.14%，所制备的丹贝冻干菌剂稳定性较好，适合菌种保存。
종배양기첨가적성분、 pH치、배양온도이급보호제등방면，연구대단패동간균제존활솔적영향，병탐구균제적은정성。결과표명，당단패균재PDA배양기중가입0.5%산리순혹0.5%맥아당항동제，배양기pH치재6.5혹8.5，31℃예배양48 h시，예동보호제채용탈지유10%，감유10%，산리순15%시，동간후균충적존활솔최고，가체73.14%，소제비적단패동간균제은정성교호，괄합균충보존。
This paper from the culture medium added ingredients,pH,culture temperature and protective agent and so on, studies effect of stem inocula survival rate of frozen to Tempe,stability and inquiry agent. Research results show that,when the Tempe bacteria in adding 0.5% sorbitol or 0.5% maltose antifreeze agent PDA in the medium,the medium pH in about 6.5 or 8.5,31 ℃pre training 48 h,pre freezing protective agent with skim milk 10%,glycerol 10%,sorbitol 15%, freeze-dried bacteria survival rate is highest,up to 73.14%. Preparation of freeze-dried inoculant Tempe is good stability, suitable for preservation.