CAJ | 학술논문

目的：探讨模拟400 m氦氧饱和潜水对大鼠肺氧化与抗氧化系统的影响。方法将SD大鼠在4．1 MPa （绝对压力）氦氧高气压环境暴露24 h，以常压空气环境作为对照。检测肺组织病理学，测定肺组织丙二醛（MDA）、8-羟基脱氧鸟苷（8-OHdG）、还原型谷胱甘肽（GSH）含量及超氧化物歧化酶（SOD）与谷胱甘肽过氧化物酶（ GSH-Px）活力。结果氦氧暴露大鼠肺组织病理学检查未见异常；肺组织匀浆中MDA、8-OHdG含量与SOD活力无明显变化，GSH-Px活力及GSH含量显著降低（P＜0．05）。结论4．1 MPa氦氧暴露24 h使大鼠肺处于氧化应激状态，抗氧化能力降低，但未造成明显的氧化损伤。
목적：탐토모의400 m양양포화잠수대대서폐양화여항양화계통적영향。방법장SD대서재4．1 MPa （절대압력）양양고기압배경폭로24 h，이상압공기배경작위대조。검측폐조직병이학，측정폐조직병이철（MDA）、8-간기탈양조감（8-OHdG）、환원형곡광감태（GSH）함량급초양화물기화매（SOD）여곡광감태과양화물매（ GSH-Px）활력。결과양양폭로대서폐조직병이학검사미견이상；폐조직균장중MDA、8-OHdG함량여SOD활력무명현변화，GSH-Px활력급GSH함량현저강저（P＜0．05）。결론4．1 MPa양양폭로24 h사대서폐처우양화응격상태，항양화능력강저，단미조성명현적양화손상。
Objective To investigate the effect of simulated 400 m He-O2 saturation diving on the pulmonary oxidative-antioxidative system in rats.Methods SD rats were exposed to 4.1 MPa He-O2 mixture for 24 h in an animal chamber. Normal air pressure was used in the control group .The histopathology , contents of malondialdehyde ( MDA) , 8-hydroxy-2′-deoxyguanosine(8-OHdG),and glutathione(GSH) and activities of superoxide dismutase (SOD) and glutathione peroxi-dase( GSH-Px) in pulmonary tissues were determined .Results No difference was found during pathological examination or in MDA, 8-OHdG and SOD activity.GSH and GSH-Px activity were decreased significantly (P<0.05) after hyperbaric exposure.Conclusion Exposure to 4.1 MPa He-O2 for 24 h may promote oxidative stress and reduce antioxidative capacity rather than cause obvious oxidative damage in rat lungs .