CAJ | 학술논문

目的：观察不同缺血时间所引起的再灌注损伤小鼠脑神经细胞Bcl-2、Caspase-3蛋白表达的变化。方法健康昆明种小鼠50只，随机均分为五组，假手术组：仅行手术，不实施脑缺血；I/RⅠ组：缺血30 min，再灌注1 h；I/RⅡ组：缺血60 min，再灌注1 h；I/RⅢ组：缺血90 min，再灌注1 h；I/RⅣ组：缺血120 min，再灌注1 h；取小鼠脑组织，进行免疫组织化学染色（ SP法）和计算机图像分析。结果在一定缺血时间范围内，Bcl-2随缺血时间的延长而表达增加，其中I/RⅡ组Bcl-2的平均光密度（MOD）值分别与假手术组和I/RⅠ组比较，均有显著性差异（P＜0.05）；但缺血时间进一步延长， I/RⅢ组和I/RⅣ组Bcl-2表达逐渐减少，分别与I/RⅡ组Bcl-2的MOD值比较，均有显著性差异（P＜0.05）。 Caspase-3随着缺血时间的延长而表达不断增加，I/RⅠ组、I/RⅡ组和I/RⅢ组Caspase-3的MOD值分别与假手术组比较，其差异均有显著性（P＜0.05）；但缺血时间过久，I/RⅣ组Caspase-3表达减弱，与I/RⅢ组Caspase-3的MOD值比较，有显著性差异（ P＜0.05）。结论在缺血早期受损神经元内， Bcl-2和 Caspase-3表达均上调且Caspase-3占优势，但随着缺血时间的进一步延长，Bcl-2表达显著减少，为临床上早期使用Caspase 抑制剂和Bcl-2激动剂治疗急性脑缺血性疾病提供实验室依据。
목적：관찰불동결혈시간소인기적재관주손상소서뇌신경세포Bcl-2、Caspase-3단백표체적변화。방법건강곤명충소서50지，수궤균분위오조，가수술조：부행수술，불실시뇌결혈；I/RⅠ조：결혈30 min，재관주1 h；I/RⅡ조：결혈60 min，재관주1 h；I/RⅢ조：결혈90 min，재관주1 h；I/RⅣ조：결혈120 min，재관주1 h；취소서뇌조직，진행면역조직화학염색（ SP법）화계산궤도상분석。결과재일정결혈시간범위내，Bcl-2수결혈시간적연장이표체증가，기중I/RⅡ조Bcl-2적평균광밀도（MOD）치분별여가수술조화I/RⅠ조비교，균유현저성차이（P＜0.05）；단결혈시간진일보연장， I/RⅢ조화I/RⅣ조Bcl-2표체축점감소，분별여I/RⅡ조Bcl-2적MOD치비교，균유현저성차이（P＜0.05）。 Caspase-3수착결혈시간적연장이표체불단증가，I/RⅠ조、I/RⅡ조화I/RⅢ조Caspase-3적MOD치분별여가수술조비교，기차이균유현저성（P＜0.05）；단결혈시간과구，I/RⅣ조Caspase-3표체감약，여I/RⅢ조Caspase-3적MOD치비교，유현저성차이（ P＜0.05）。결론재결혈조기수손신경원내， Bcl-2화 Caspase-3표체균상조차Caspase-3점우세，단수착결혈시간적진일보연장，Bcl-2표체현저감소，위림상상조기사용Caspase 억제제화Bcl-2격동제치료급성뇌결혈성질병제공실험실의거。
Objective To observe the expression and significance of Bcl-2 and Caspase-3 in brain neurons cells of the different is-chemia reperfusion injury of mice and for the treatment and prognosis of acute cerebral ischemic diseases clinically provide laboratory basis . Methods 50 healthy Kunming mice were randomly divided into the following 5 groups:control group:only with surgery , not implement cer-ebral ischemia;ischemia-reperfusion groupⅠ( I/RⅠgroup ) , 30 min of ischemia and reperfusion 1 h; ischemia-reperfusion Ⅱ groups:60 min of ischemia and reperfusion 1 h group ( I/RⅡgroup);ischemia-reperfusion Ⅲgroup:90 min of ischemia and reperfusion 1 h group ( I/RⅢgroup);ischemia-reperfusion Ⅳgroup:120 min of ischemia and reperfusion 1 h group (I/RⅣgroup).Then all mice were killed and the brain glands were taken for immunohistochemistry staining and image analysis by computer .Results ①The result of immunohistochem-isty:the expression of Bcl-2 and Caspase-3 was pale brown in the brain neurons cells of mice of fasle operation group;the expression of Bcl-2 and Caspase-3 was brown yellow of I/R group;the expression of Bcl-2 and Caspase-3 was brown of I/R Ⅱgroup;compared with I/R Ⅱgroup, the expression of Bcl-2 was reduced significantly in I/R Ⅲgroup and I/RⅣgroup.The expression of Caspase-3 was dark brown in the neurons cells of mice of I/RⅢgroup;the expression of Caspase-3 was brown in I/RⅣgroup.②Comparison between the MOD of Bcl-2 in sham operation group and that of I/RⅡgroup was significant (P<0.05).The expression of Bcl-2 was significantly enhanced than that of I/R Ⅰand Ⅲgroup(P<0.05), the difference of between the MOD of Bcl-2 in I/RⅡgroup and that of I/RⅣgroup was significant(P<0.05).The MOD of Caspase-3 in I/RⅠgroup,I/RⅡgroup, I/RⅢgroup and I/RⅣgroup were compared with that of sham operation group , there were significant differences (P<0.05).the MOD of Caspase-3 in I/R Ⅱgroup and I/R Ⅲgroup were compared with that of I/RⅠgroup, there were significant differences (P<0.05); the difference of between the MOD of Caspase-3 in I/R Ⅱgroup and that of I/R Ⅲgroup was significant(P<0.05).The difference of Caspase-3 in I/RⅢgroup and that of I/RⅣgroup was significant(P<0.05).Conclu-sions The expression of Caspase-3 is increased significantly than that of Bcl-2 in the impaired early ischemic neurons .However, as the is-chemia time delay further , the expression of Bcl-2 is first reduced significantly , and laboratory basis is further provided for therapy early in a-cute cerebral ischemic diseases with Caspase inhibitors and Bcl-2 agonist .