CAJ | 학술논문

目的：探索氢饱和盐水对阿尔茨海默病（AD）模型大鼠氧化应激的影响。方法选用280～320 g Sprague-Dawley（SD）大鼠，随机分为模型组、假手术组、氢饱和盐水治疗组；利用立体定向技术侧脑室注射Aβ1～42建立AD模型大鼠，腹腔注射氢饱和盐水。给药14 d后采用生物化学法检测各组大鼠的脑海马组织匀浆的MDA含量，利用免疫组织化学法检测各组大鼠脑海马组织氧化应激标志物HNE及8-OH-d G蛋白的表达。结果模型组大鼠脑组织MDA含量为（6.65±1.07）nmol/mgprotein，与假手术组相比显著升高（P＝0.000），而氢饱和盐水治疗组大鼠脑组织的MDA含量较模型组大鼠显著降低（ P＝0.016）。模型组大鼠海马齿状回显现较多的HNE阳性细胞，假手术组大鼠鲜见HNE阳性细胞，而氢饱和盐水治疗组大鼠海马齿状回HNE阳性细胞较模型组大鼠显著减少（P＝0.011）。海马齿状回呈现棕黄色8-OH-d G染色阳性细胞，与假手术组大鼠相比，模型组大鼠海马齿状回出现明显增多的8-OH-d G染色阳性细胞（P＝0.000），而经氢饱和盐水治疗后的模型大鼠海马齿状回8-OH-d G 染色阳性细胞明显减少（P＝0.000）。顶叶皮层呈现棕黄色8-OH-d G染色阳性细胞，与假手术组大鼠相比，模型组大鼠顶叶皮层出现明显增多的8-OH-d G染色阳性细胞（P＝0.000），而经氢饱和盐水治疗后的模型大鼠顶叶皮层8-OH-d G染色阳性细胞明显减少（P＝0.000）。结论氢饱和盐水可显著减少Aβ诱导的氧化应激损伤蛋白及核酸标志物的表达。
목적：탐색경포화염수대아이자해묵병（AD）모형대서양화응격적영향。방법선용280～320 g Sprague-Dawley（SD）대서，수궤분위모형조、가수술조、경포화염수치료조；이용입체정향기술측뇌실주사Aβ1～42건립AD모형대서，복강주사경포화염수。급약14 d후채용생물화학법검측각조대서적뇌해마조직균장적MDA함량，이용면역조직화학법검측각조대서뇌해마조직양화응격표지물HNE급8-OH-d G단백적표체。결과모형조대서뇌조직MDA함량위（6.65±1.07）nmol/mgprotein，여가수술조상비현저승고（P＝0.000），이경포화염수치료조대서뇌조직적MDA함량교모형조대서현저강저（ P＝0.016）。모형조대서해마치상회현현교다적HNE양성세포，가수술조대서선견HNE양성세포，이경포화염수치료조대서해마치상회HNE양성세포교모형조대서현저감소（P＝0.011）。해마치상회정현종황색8-OH-d G염색양성세포，여가수술조대서상비，모형조대서해마치상회출현명현증다적8-OH-d G염색양성세포（P＝0.000），이경경포화염수치료후적모형대서해마치상회8-OH-d G 염색양성세포명현감소（P＝0.000）。정협피층정현종황색8-OH-d G염색양성세포，여가수술조대서상비，모형조대서정협피층출현명현증다적8-OH-d G염색양성세포（P＝0.000），이경경포화염수치료후적모형대서정협피층8-OH-d G염색양성세포명현감소（P＝0.000）。결론경포화염수가현저감소Aβ유도적양화응격손상단백급핵산표지물적표체。
Objective To explore the effect of hydrogen-rich improving learning and memory abilities of a rat model of Alzheimer's disease(AD).Methods S-D male rats (n=84, 280~330 g) were divided into sham-operated, Aβ1~42injected and Aβ1~42 plus hydrogen-rich saline-treated groups.Hydrogen-rich saline (5 ml/kg, i.p., daily) was injected for 14 days after intracerebroventricular injection of Aβ1~42.Morris water Maze and open field task were used to assess the memory dysfunction and motor dysfunction , respectively.LTP was used to detect the electrophysiology changes.Results In training trials, the escape latency time on the last training day (the fifth day) was (10.64±8.62) s in sham group, (30.10±20.93) s in Aβ1~42 plus physiological saline group, and (18.14±16.16) s in Aβ1~42 plus hydro-gen-rich saline group.In probe trials, animals in hydrogen-rich saline group spent more time on the right quadrant for searching the platform when compared with that of Aβ1~42 plus physiological saline group ( P=0.026 ).No alterations of the swimming speed ( F=1.73;P=0.18 ) in the Morris water Maze , or the total squares crossed ( F=0.81;P=0.45 ) and rearing behavior ( F=0.62;P=0.54 ) in the open field are-na were observed.In sham group , 200 Hz conditioning stimulation induced a robust and stable LTP of the EPSP amplitude compared to pre -HFS baseline〔(177±5)%of baseline at 1 h post-HFS〕, and the LTP was produced in the Aβ1~42(2.2 nmol) group〔(127±6)%〕following HFS( P=0.000 ) , and which was attenuated significantly in hydrogen-rich saline group〔( 148 ±8 )%〕( P=0.000 ) .Conclusions Hydro-gen-rich saline could improve amyloid β( Aβ) induced learning and memory deficits of AD model rats .