CAJ | 학술논문

目的：探析感染EV71重症手足口病细胞因子水平与免疫球蛋白的应用研究。方法回顾性调查我院2012年1-12月EV71感染的手足口病神经系统受累期患者104例，分为两组，每组52例，A组于入院当天及次日予以静脉内注射免疫球蛋白（IVIG）1g/kg治疗；B组未予IVIG疗法，另选择18例健康同龄儿童作为正常对照组，应用ELISA法检测所有研究对象的IL-6、IL-10、TNF-α、TGF-β血清浓度，比较EV71感染手足口病患儿IVIG治疗前后的细胞因子水平变化。结果重症手足口病患儿应用IVIG后血清IL-6、IL-10、TNF-α明显下降，结果具有统计学意义（ P＜0．05），而TGF-β没有显著差异。应用IVIG可缩短住院时间（P＜0．05），但是治愈率、死亡率没有显著差异。结论重症EV71感染手足口病患儿免疫功能变化复杂，应用IVIG可调节细胞因子水平，从而达到抗炎症反应和调节免疫功能的作用，缩短住院时间。
목적：탐석감염EV71중증수족구병세포인자수평여면역구단백적응용연구。방법회고성조사아원2012년1-12월EV71감염적수족구병신경계통수루기환자104례，분위량조，매조52례，A조우입원당천급차일여이정맥내주사면역구단백（IVIG）1g/kg치료；B조미여IVIG요법，령선택18례건강동령인동작위정상대조조，응용ELISA법검측소유연구대상적IL-6、IL-10、TNF-α、TGF-β혈청농도，비교EV71감염수족구병환인IVIG치료전후적세포인자수평변화。결과중증수족구병환인응용IVIG후혈청IL-6、IL-10、TNF-α명현하강，결과구유통계학의의（ P＜0．05），이TGF-β몰유현저차이。응용IVIG가축단주원시간（P＜0．05），단시치유솔、사망솔몰유현저차이。결론중증EV71감염수족구병환인면역공능변화복잡，응용IVIG가조절세포인자수평，종이체도항염증반응화조절면역공능적작용，축단주원시간。
Objective Analyze the levels of cytokines in patients who infected severe-hand-foot mouth disease and the application of immunoglobulin .Methods Retrospectively investigated the datas of 104 cases of patients who were suffered from hand-foot-mouth disease and got therapy in our hospital during January 2012 and December 2012, they were divided into two groups , 52 cases in each group.Patients in group A were treated with immunoglobulin ( IVIG) 1g/kg through intravenous access on admission day and the next day , and the treatment for patients in group B without IVIG , another 18 cases healthy children served as normal control group .ELISA assay was applied to detect the concentration of cytokines (IL-6, IL-10, TNF-α, TGF-β) in serum, cytokine levels in children with hand-foot-mouth disease were compared .Results Concentration of IL-6, IL-10, TNF-αin serum of HFMD patients decreased significantly after the application of IVIG (P<0.05), but the level of TGF-βdidn't change.Application of IVIG can shorten the time of hospitalization (P<0.05), but cure rate and mortality rate showed no significant change .Conclusions The changes of immune function in children with hand foot and mouth disease were complex , and the application of IVIG can regulate levels of cell factor and achieve the purpose of anti-inflammatory reaction and regulating immune function ,shorten the time of hospitalization .