华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2014年
4期
413-417
,共5页
黄瑾%刘建国%宋琦%苏牧%张疆弢%管晓燕%彭菊香
黃瑾%劉建國%宋琦%囌牧%張疆弢%管曉燕%彭菊香
황근%류건국%송기%소목%장강도%관효연%팽국향
转化生长因子-β1%血小板衍生生长因子-BB%整合素β3%正畸牙牙周组织
轉化生長因子-β1%血小闆衍生生長因子-BB%整閤素β3%正畸牙牙週組織
전화생장인자-β1%혈소판연생생장인자-BB%정합소β3%정기아아주조직
transforming growth factor-β1%platelet-derived growth factor-BB%integrin β3%periodontal tissue of orthodontic tooth
目的:观察大鼠正畸牙局部联合应用血小板衍生生长因子-BB(PDGF-BB)和转化生长因子-β1(TGF-β1)后正畸牙牙周膜中整合素β3表达的变化。方法按随机数字表法,将32只大鼠随机均分为4组。建立大鼠上颌第一磨牙近中移动正畸模型,隔日于施力磨牙颊侧牙龈黏膜下分别注射1%PBS、10 ng PDGF-BB、5 ng TGF-β1、10 ng PDGF-BB和5 ng TGF-β1叠加剂量,注射体积均为0.1 mL。加力10 d后处死动物,取左上颌第一磨牙及其牙周组织,用免疫组织化学方法检测牙周组织中整合素β3的表达,进行阳性表达区域平均光密度值测量,对测量结果行方差分析,服从正态分布,再行组间t检验统计学分析。结果各实验组压力侧牙周膜细胞中整合素β3的表达强度均高于对照组(P<0.01),其中PDGF-BB+TGF-β1组与TGF-β1组或PDGF-BB组比较差异均有统计学意义(P<0.01)。实验组张力侧牙周膜细胞中整合素β3的表达强度均高于对照组(P<0.05),其中PDGF-BB+TGF-β1组整合素β3表达强度较强,与TGF-β1或PDGF-BB组比较差异无统计学意义(P>0.05)。结论局部联合应用外源性PDGF-BB和TGF-β1对大鼠正畸牙牙周组织中整合素β3的上调作用较单独运用PDGF-BB或TGF-β1组强,二者联合应用有协同效应,共同促进了正畸牙周组织改建。
目的:觀察大鼠正畸牙跼部聯閤應用血小闆衍生生長因子-BB(PDGF-BB)和轉化生長因子-β1(TGF-β1)後正畸牙牙週膜中整閤素β3錶達的變化。方法按隨機數字錶法,將32隻大鼠隨機均分為4組。建立大鼠上頜第一磨牙近中移動正畸模型,隔日于施力磨牙頰側牙齦黏膜下分彆註射1%PBS、10 ng PDGF-BB、5 ng TGF-β1、10 ng PDGF-BB和5 ng TGF-β1疊加劑量,註射體積均為0.1 mL。加力10 d後處死動物,取左上頜第一磨牙及其牙週組織,用免疫組織化學方法檢測牙週組織中整閤素β3的錶達,進行暘性錶達區域平均光密度值測量,對測量結果行方差分析,服從正態分佈,再行組間t檢驗統計學分析。結果各實驗組壓力側牙週膜細胞中整閤素β3的錶達彊度均高于對照組(P<0.01),其中PDGF-BB+TGF-β1組與TGF-β1組或PDGF-BB組比較差異均有統計學意義(P<0.01)。實驗組張力側牙週膜細胞中整閤素β3的錶達彊度均高于對照組(P<0.05),其中PDGF-BB+TGF-β1組整閤素β3錶達彊度較彊,與TGF-β1或PDGF-BB組比較差異無統計學意義(P>0.05)。結論跼部聯閤應用外源性PDGF-BB和TGF-β1對大鼠正畸牙牙週組織中整閤素β3的上調作用較單獨運用PDGF-BB或TGF-β1組彊,二者聯閤應用有協同效應,共同促進瞭正畸牙週組織改建。
목적:관찰대서정기아국부연합응용혈소판연생생장인자-BB(PDGF-BB)화전화생장인자-β1(TGF-β1)후정기아아주막중정합소β3표체적변화。방법안수궤수자표법,장32지대서수궤균분위4조。건립대서상합제일마아근중이동정기모형,격일우시력마아협측아간점막하분별주사1%PBS、10 ng PDGF-BB、5 ng TGF-β1、10 ng PDGF-BB화5 ng TGF-β1첩가제량,주사체적균위0.1 mL。가력10 d후처사동물,취좌상합제일마아급기아주조직,용면역조직화학방법검측아주조직중정합소β3적표체,진행양성표체구역평균광밀도치측량,대측량결과행방차분석,복종정태분포,재행조간t검험통계학분석。결과각실험조압력측아주막세포중정합소β3적표체강도균고우대조조(P<0.01),기중PDGF-BB+TGF-β1조여TGF-β1조혹PDGF-BB조비교차이균유통계학의의(P<0.01)。실험조장력측아주막세포중정합소β3적표체강도균고우대조조(P<0.05),기중PDGF-BB+TGF-β1조정합소β3표체강도교강,여TGF-β1혹PDGF-BB조비교차이무통계학의의(P>0.05)。결론국부연합응용외원성PDGF-BB화TGF-β1대대서정기아아주조직중정합소β3적상조작용교단독운용PDGF-BB혹TGF-β1조강,이자연합응용유협동효응,공동촉진료정기아주조직개건。
Objective To investigate the synergistic effect of transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor-BB (PDGF-BB) on the expression of integrin β3 in periodontal membrane of rat orthodontic tooth. Methods An orthodontic tooth movement model was established. Up to 32 experimental rats were randomly divided into four groups according to a random number table. The four groups were injected with 1%PBS, TGF-β1 (5 ng), PDGF-BB (10 ng), and combined TGF-β1 (5 ng) and PDGF-BB (10 ng) in the buccal submucosal, respectively. The volume injected in each group was 0.1 mL. The animals were then sacrificed on the 10th day. The left maxillary first molar and periodontal tissue were taken. Different expressions of integrin β3 were detected in periodontal tissues through immunohistochemistry. Mean optical density (OD) values of the positive fields were examined. The data obtained were analyzed through ANOVA. The data followed normal distribution, and were compared via t-test. Results Compared with the control groups, the expression of integrin β3 was higher in the experimental groupin tension sides (P<0.01). Significant differences in tension sides between the singleinjection groups and the combined group were observed (P<0.01). Compared with the control groups, the expression of integrin β3 was higher in the experimental group in compression sides (P<0.05). In addition, there was no significant differences in compression sides between the single-injection groups and the combined group (P>0.05). Conclusion In terms of local regulatory factors, TGF-β1 combined with PDGF-BB enhance the expression of integrin β3 in the periodontal membrane and accelerate periodontal remodeling. The synergistic effect of the two growth factors is better than the single growth factor.