CAJ | 학술논문

目的：研究含不同浓度碘（I-）的乳过氧化物酶-过氧化氢-硫氰化物系统（LPO-H2O2-SCN-）对变异链球菌生长、黏附、产水不溶性细胞外多糖以及葡萄糖基转移酶（GTF）活性的影响。方法选用S. mutans ATCC 25175为实验菌株。采用菌落形成单位（CFU）计数法进行生长实验；用分光光度计法测定细菌的黏附抑制率；用蒽酮法测定水不溶性细胞外多糖的质量浓度；用蒽酮法测定还原糖量，计算GTF活性。结果随着I-浓度的增高，含I-的LPO-H2O2-SCN-抗菌系统对S. mutans致龋力的抑制作用增强。当I-≥100μmol·L-1时，对S. mutans的生长抑制明显高于对照组（P<0.05）；且当I-≥1000μmol·L-1时，对S. mutans的生长抑制显著高于以硫氰酸盐（SCN-）为单底物的实验组（P<0.05）；当I-≥100μmol·L-1时，对S. mutans的黏附抑制率达到50%以上，水不溶性细胞外多糖生成量明显减少（P<0.05），GTF活性也明显降低（P<0.05）。结论通过增加I-的浓度，可以抵消生理浓度的SCN-的抑制作用，使含I-的LPO-H2O2-SCN-系统能显著抑制变异链球菌的生长、黏附、水不溶性细胞外多糖生成和GTF活性。
목적：연구함불동농도전（I-）적유과양화물매-과양화경-류청화물계통（LPO-H2O2-SCN-）대변이련구균생장、점부、산수불용성세포외다당이급포도당기전이매（GTF）활성적영향。방법선용S. mutans ATCC 25175위실험균주。채용균락형성단위（CFU）계수법진행생장실험；용분광광도계법측정세균적점부억제솔；용은동법측정수불용성세포외다당적질량농도；용은동법측정환원당량，계산GTF활성。결과수착I-농도적증고，함I-적LPO-H2O2-SCN-항균계통대S. mutans치우력적억제작용증강。당I-≥100μmol·L-1시，대S. mutans적생장억제명현고우대조조（P<0.05）；차당I-≥1000μmol·L-1시，대S. mutans적생장억제현저고우이류청산염（SCN-）위단저물적실험조（P<0.05）；당I-≥100μmol·L-1시，대S. mutans적점부억제솔체도50%이상，수불용성세포외다당생성량명현감소（P<0.05），GTF활성야명현강저（P<0.05）。결론통과증가I-적농도，가이저소생리농도적SCN-적억제작용，사함I-적LPO-H2O2-SCN-계통능현저억제변이련구균적생장、점부、수불용성세포외다당생성화GTF활성。
Objective This study aimed to investigate the effect of a lactoperoxidase-peroxidase-thiocyanate (LPO-H2O2- SCN-) system with different concentrations of iodine (I-) on Streptococcus mutans (S. mutans), particularly on various parameters, including growth, adhesion, glucosyltransferase (GTF) enzyme activity, and insoluble exopolysaccharide synthesis. Methods S. mutans ATCC 25175 was used as experimental species. Clonal formation unit (CFU) were counted to investigate the inhibitory effect on bacterial growth. The inhibition rate of bacterial adherence was calculated to analyze the effect on adhesion. Anthrone method was used to determine the content of insoluble exopolysaccharides and the amount of reducing saccharides. GTF activity and enzyme activity were then determined. Results The inhibitory ability of the LPO-H2O2-SCN- system with I- on the cariogenicinity of S. mutans was strengthened as I- concentration was increased. At I- concentration≥100 μmol·L-1, the antibacterial effects were significantly increased compared with those of the control group (P<0.05). At I- concentration≥ 1 000 μmol·L-1, the antibacterial effects were significantly improved compared with those of the group with SCN- only (P< 0.05). At I- concentration≥100 μmol·L-1, the inhibition rate of bacterial adherence was>50%; insoluble exopolysaccharide synthesis and GTF enzyme activity were reduced (P<0.05). Conclusion The antibacterial effects of the LPO-H2O2-I- system were enhanced by adding I- to overcome the antagonistic effect of physiological SCN- concentration. LPO-H2O2-SCN- system with different concentrations of I- showed statistically significant inhibitory effects on growth, adhesion, insoluble exopolysaccharide synthesis, and GTF enzyme activity.