CAJ | 학술논문

目的：探索改善钛种植体表面处理的新方法，提高种植体植入后成骨效率。方法将RGD肽与壳聚糖（CS）通过酰化反应发生偶联形成RGD-CS，采用复凝聚法制备RGD-CS/pDNA复合体，并将RGD-CS/pDNA复合体接枝到经物理、生化处理后的纯钛表面。采用红外光谱仪和元素分析仪对RGD-CS进行化学结构的表征检测，凝胶电泳阻滞试验结合原子力显微镜观察RGD-CS对质粒的包裹情况及RGD-CS/pDNA复合体的形态，EB染色法检测钛片表面RGD-CS/pDNA复合体的接枝效果。结果红外光谱检测结合元素分析表明，CS和RGD肽偶联成功；凝胶电泳阻滞试验结合原子力显微镜观察表明，在N/P≥2时，RGD-CS与pDNA完全复合，RGD-CS/pDNA复合体呈类球形；EB染色表明RGD-CS/pDNA复合体接枝钛片成功。结论经RGD肽修饰的壳聚糖可以携带pDNA作为钛种植体表面质粒包装的载体。
목적： 탐색개선태충식체표면처리적신방법，제고충식체식입후성골효솔。방법 장RGD태여각취당（CS）통과선화반응발생우련형성RGD-CS，채용복응취법제비RGD-CS/pDNA복합체，병장RGD-CS/pDNA복합체접지도경물리、생화처리후적순태표면。채용홍외광보의화원소분석의대RGD-CS진행화학결구적표정검측，응효전영조체시험결합원자력현미경관찰RGD-CS대질립적포과정황급RGD-CS/pDNA복합체적형태，EB염색법검측태편표면RGD-CS/pDNA복합체적접지효과。결과 홍외광보검측결합원소분석표명，CS화RGD태우련성공；응효전영조체시험결합원자력현미경관찰표명，재N/P≥2시，RGD-CS여pDNA완전복합，RGD-CS/pDNA복합체정류구형；EB염색표명RGD-CS/pDNA복합체접지태편성공。결론 경RGD태수식적각취당가이휴대pDNA작위태충식체표면질립포장적재체。
Objective This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency. Methods An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS. An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed. The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer. The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy. The grafting effect of this complex on the titanium surface was detected by EB staining. Results CS and RGD peptides were coupled by an amide bond. The RGD-CS/pDNA complex was completely composited at N/P≥2. Atomic force microscopy results showed that the morphology of this complex was mainly spherical. EB staining experiments showed that this complex was successfully grafted on the titanium plate. Conclusion RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.