药学与临床研究
藥學與臨床研究
약학여림상연구
PHARMACEUTICAL AND CLINICAL RESEARCH
2014年
4期
336-338
,共3页
闫文静%仲彦颖%汪豪%李萍
閆文靜%仲彥穎%汪豪%李萍
염문정%중언영%왕호%리평
川牛膝%三萜皂苷%川牛膝皂苷A%B%HPLC-ELSD法%含量测定
川牛膝%三萜皂苷%川牛膝皂苷A%B%HPLC-ELSD法%含量測定
천우슬%삼첩조감%천우슬조감A%B%HPLC-ELSD법%함량측정
Cyathula officinalis%Triterpenoid saponins%Cyanoside A,B%HPLC-ELSD method%Quantita-tive analysis
目的:建立川牛膝中2种主要三萜皂苷---川牛膝皂苷A、B的含量测定方法。方法:采用HPLC-ELSD法,Shim-pack VP-ODS C18(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-0.1%甲酸水(37∶63);体积流量为1.0 mL·min-1;ELSD漂移管温度为105℃;载气流速2.8 L· min-1。结果:3-O-{β-D-吡喃葡萄糖-(1→2)-[α-L-吡喃鼠李糖-(1→3)]-β-D-吡喃葡萄糖醛酸基}齐墩果酸-28-O-β-D-吡喃葡萄糖苷(川牛膝皂苷A)和3-O-[α-L-吡喃鼠李糖-(1→3)-β-D-吡喃葡萄糖醛酸基]齐墩果酸-28-O-β-D-吡喃葡萄糖苷(川牛膝皂苷B)的含量分别为0.47%~1.37%和0.46%~1.32%;线性范围分别为1.0~20.0μg与0.8~16.0μg;平均回收率分别为99.72%和97.72%。结论:HPLC-ELSD含量测定方法准确、简便、专属性好,适用于川牛膝的质量评价。
目的:建立川牛膝中2種主要三萜皂苷---川牛膝皂苷A、B的含量測定方法。方法:採用HPLC-ELSD法,Shim-pack VP-ODS C18(4.6 mm×250 mm,5μm)色譜柱;流動相為乙腈-0.1%甲痠水(37∶63);體積流量為1.0 mL·min-1;ELSD漂移管溫度為105℃;載氣流速2.8 L· min-1。結果:3-O-{β-D-吡喃葡萄糖-(1→2)-[α-L-吡喃鼠李糖-(1→3)]-β-D-吡喃葡萄糖醛痠基}齊墩果痠-28-O-β-D-吡喃葡萄糖苷(川牛膝皂苷A)和3-O-[α-L-吡喃鼠李糖-(1→3)-β-D-吡喃葡萄糖醛痠基]齊墩果痠-28-O-β-D-吡喃葡萄糖苷(川牛膝皂苷B)的含量分彆為0.47%~1.37%和0.46%~1.32%;線性範圍分彆為1.0~20.0μg與0.8~16.0μg;平均迴收率分彆為99.72%和97.72%。結論:HPLC-ELSD含量測定方法準確、簡便、專屬性好,適用于川牛膝的質量評價。
목적:건립천우슬중2충주요삼첩조감---천우슬조감A、B적함량측정방법。방법:채용HPLC-ELSD법,Shim-pack VP-ODS C18(4.6 mm×250 mm,5μm)색보주;류동상위을정-0.1%갑산수(37∶63);체적류량위1.0 mL·min-1;ELSD표이관온도위105℃;재기류속2.8 L· min-1。결과:3-O-{β-D-필남포도당-(1→2)-[α-L-필남서리당-(1→3)]-β-D-필남포도당철산기}제돈과산-28-O-β-D-필남포도당감(천우슬조감A)화3-O-[α-L-필남서리당-(1→3)-β-D-필남포도당철산기]제돈과산-28-O-β-D-필남포도당감(천우슬조감B)적함량분별위0.47%~1.37%화0.46%~1.32%;선성범위분별위1.0~20.0μg여0.8~16.0μg;평균회수솔분별위99.72%화97.72%。결론:HPLC-ELSD함량측정방법준학、간편、전속성호,괄용우천우슬적질량평개。
To establish a quantitative analysis method of the triterpenoid saponins in Cy-athula officinalis. Methods: An HPLC-ELSD method was used to determine the contents of two major triterpenoid saponins in C. officinalis. The separation was performed on a Shim-pack VP-ODS column (C18, 5μm, 4.6 mm×250 mm), with a mixture solution of acetonitrile-0.1% formic acid in water (37∶63) as mobile phase. The flow rate was 1.0 mL·min-1. The drift tube temperature of ELSD detector was 105℃, the gas flow was 2.8 L·min-1. Results: The contents of 3-O-{β-D-glucopy- ranosyl-(1→2)-[α-L-rhamnopyanosyl-(1→3)]-(β-D-glucuronopyranosyl)}-28-O-β-D-glucopyranosyl oleanolic acid (cyaonoside A) and 3-O-α-L-rhamnopyranosyl-(1→3)- β-D-glucuronopyranosyl-28-O-β-D-glucopyranosyl oleanolic acid (cyaonoside B) were 0.47%~1.37% and 0.46%~1.32%, respectively. The linear range of were 1.0~20.0μg and 0.8~16.0μg and the average recoveries were 99.72% and 97.72%, respectively. Conclusion: The HPLC-ELSD method is accurate and suitable for the quantitative analysis of these two saponins in C. officinalis.