CAJ | 학술논문

目的：研究中药在体外对痤疮致病菌的抑制作用，筛选用于治疗痤疮的中药。方法：制备20种中药提取物，采用平板法37℃培养金黄色葡萄球菌和痤疮丙酸杆菌，通过2倍稀释法定量测定20味中药和6种中药单体成分最低抑菌浓度（MIC）。结果：20种中药提取物中大黄、黄芩、甘草、牡丹皮、金银花5种中药对两种致病菌均有抑菌作用，其中对金黄色葡萄球菌的MIC分别为6.25 mg·mL-1、6.25 mg·mL-1、12.5 mg·mL-1、12.5 mg·mL-1、12.5 mg·mL-1，对痤疮丙酸杆菌的MIC分别为12.5 mg·mL-1、12.5 mg·mL-1、50 mg·mL-1、50 mg·mL-1、50 mg·mL-1。6种中药单体成分中大黄酸、黄芩苷对两种致病菌抑菌作用最佳，对金黄色葡萄球菌的MIC分别为15.625μg·mL-1、7.812μg·mL-1，对痤疮丙酸杆菌的MIC分别为31.25μg·mL-1、15.625μg·mL-1。结论：大黄、黄芩、甘草、牡丹皮、金银花和单体成分大黄酸、黄芩苷表现出良好的抑菌效果，在进一步的药物筛选中可进行重点考察。
목적：연구중약재체외대좌창치병균적억제작용，사선용우치료좌창적중약。방법：제비20충중약제취물，채용평판법37℃배양금황색포도구균화좌창병산간균，통과2배희석법정량측정20미중약화6충중약단체성분최저억균농도（MIC）。결과：20충중약제취물중대황、황금、감초、모단피、금은화5충중약대량충치병균균유억균작용，기중대금황색포도구균적MIC분별위6.25 mg·mL-1、6.25 mg·mL-1、12.5 mg·mL-1、12.5 mg·mL-1、12.5 mg·mL-1，대좌창병산간균적MIC분별위12.5 mg·mL-1、12.5 mg·mL-1、50 mg·mL-1、50 mg·mL-1、50 mg·mL-1。6충중약단체성분중대황산、황금감대량충치병균억균작용최가，대금황색포도구균적MIC분별위15.625μg·mL-1、7.812μg·mL-1，대좌창병산간균적MIC분별위31.25μg·mL-1、15.625μg·mL-1。결론：대황、황금、감초、모단피、금은화화단체성분대황산、황금감표현출량호적억균효과，재진일보적약물사선중가진행중점고찰。
To select proper traditional Chinese medicines (TCM) for acne, according to their in vitro antimicrobial activities. Methods: An agar plate dilution method was used to determine the mini-mal inhibitory concentration (MIC) of the water extracts of 20 kinds of traditional Chinese medicines and 6 kinds of TCM compounds, respectively, to Staphylococcus aureus and Propionibacterium acnes. Result: The TCM which had inhibitory functions for both bacterias were Rhei Radix et Rhizoma, Radix Scutellariae, Glycyrrhizae, Moutan Cortex, Lonicerae Japonicae Flos, rheinic acid and baicalin. The MICs were 6.25 mg· mL-1, 6.25 mg·mL-1, 12.5 mg·mL-1, 12.5 mg·mL-1, 12.5 mg·mL-1, 15.625 μg·mL-1 and 7.812 μg·mL-1 to Staphylococcus aureus and 12.5 mg·mL-1, 12.5 mg·mL-1, 50 mg·mL-1, 50 mg·mL-1, 50 mg·mL-1, 31.25μg· mL-1 and 15.625μg·mL-1 to Propionibacterium acnes, respectively. Conclusion: More attention should be paid to Rhei Radix et Rhizoma, Radix Scutellariae, Glycyrrhizae, Moutan Cortex, Lonicerae Japonicae Flos, rheinic acid and baicalin in further studies.