CAJ | 학술논문

在模拟生理条件下,利用荧光光谱和同步荧光光谱法研究 Meso-四(对-羟基苯基)卟啉(THPP)与溶菌酶的相互作用.结果表明：THPP 对溶菌酶的猝灭过程为静态猝灭；291 K时,THPP 与溶菌酶的结合常数为5.97×104 L/mol；疏水作用力为 THPP 与溶菌酶的主要作用力；金属离子 Fe2+,Fe3+,Cu2+,Mg2+,Ca2+和 Zn2+的存在不影响 THPP 与溶菌酶的结合常数；THPP 与溶菌酶的结合距离为3.35 nm；THPP 可使色氨酸残基周围的极性减弱,疏水性增强.
재모의생리조건하,이용형광광보화동보형광광보법연구 Meso-사(대-간기분기)계람(THPP)여용균매적상호작용.결과표명：THPP 대용균매적졸멸과정위정태졸멸；291 K시,THPP 여용균매적결합상수위5.97×104 L/mol；소수작용력위 THPP 여용균매적주요작용력；금속리자 Fe2+,Fe3+,Cu2+,Mg2+,Ca2+화 Zn2+적존재불영향 THPP 여용균매적결합상수；THPP 여용균매적결합거리위3.35 nm；THPP 가사색안산잔기주위적겁성감약,소수성증강.
Meso-tetra(4-hydroxyphenyl)porphyrin (THPP)interacting with lysozyme was investigated by the fluorescence spectroscopy under simulated physiological conditions.The results indicate that THPP quenched the intrinsic fluorescence of lysozyme via a static quenching procedure.The binding constant KA between THPP and lysozyme was 5.97 × 10 4 L/mol at 291 K.According to the thermodynamic parameters,it was obtained that hydrophobic was a main acting force between THPP and lysozyme.The presence of Fe2 + ,Fe3 + ,Cu2 + ,Mg2 + ,Ca2 + and Zn2 + did not affect the binding constant of THPP and lysozyme.According to F?rster’s non-radiative energy transfer theory,the distance between THPP and lysozyme was 3.35 nm.The synchronous fluorescence spectra reveal that the vicinity of tryptophan becomes more hydrophobicity with the increase of THPP.