CAJ | 학술논문

QTL 的精确定位对数量性状遗传机制的研究及应用具有重要意义。随着分子生物技术和遗传学的发展，产生了大量可用于 QTL 定位的遗传标记和分析方法。然而，多数方法只能将 QTL 定位到一个区间而非具体位置，且具有较高的假阳性。为此，提出利用全基因组上的 SNP 标记，通过 emBayesB 方法和性状-标记回归区间分析相结合的组合方法进行 QTL 定位研究。组合方法能够筛选出与 QTL 存在较强关联的 SNP 标记，具有较高的计算速度和计算效率；通过性状-标记区间检测，能够较为精确地计算出 QTL 的位置。方法中考虑到染色体上其它标记的背景遗传信息，提高定位成功率和可信度。
QTL 적정학정위대수량성상유전궤제적연구급응용구유중요의의。수착분자생물기술화유전학적발전，산생료대량가용우 QTL 정위적유전표기화분석방법。연이，다수방법지능장 QTL 정위도일개구간이비구체위치，차구유교고적가양성。위차，제출이용전기인조상적 SNP 표기，통과 emBayesB 방법화성상-표기회귀구간분석상결합적조합방법진행 QTL 정위연구。조합방법능구사선출여 QTL 존재교강관련적 SNP 표기，구유교고적계산속도화계산효솔；통과성상-표기구간검측，능구교위정학지계산출 QTL 적위치。방법중고필도염색체상기타표기적배경유전신식，제고정위성공솔화가신도。
Search the precise position of QTL ,is very significant for understanding the genetic mechanisms of quantitative traits and its application.The development of molecular biology and genetics results in a large number of genetic markers and analytical methods can be used for QTL mapping.However,most methods can only map QTL to a interval rather than a specific location with a high false positive.This paper proposes that the use of genome -wide SNP markers and combina-tion strategy which combining emBayesB methods and traits - mark regression interval analysis conducted QTL mapping studies.Combination strategy could screen out SNP markers which associated with QTL in the presence of strong,and hav-ing a high computing speed and efficiency;through trait -mark interval detection,the location of the QTL can be calculat-ed more accurately.The method takes into account the background genetic information of other genetic markers on the chro-mosome,achieves higher position success rate,and improves the positioning reliability.