茶叶科学
茶葉科學
다협과학
2014年
4期
401-407
,共7页
张聪%谭蓉%孔俊豪%葛庆丰%杨秀芳%汪志君
張聰%譚蓉%孔俊豪%葛慶豐%楊秀芳%汪誌君
장총%담용%공준호%갈경봉%양수방%왕지군
茶黄素%儿茶素%绞股蓝皂苷%体外%降血脂
茶黃素%兒茶素%絞股藍皂苷%體外%降血脂
다황소%인다소%교고람조감%체외%강혈지
theaflavins%catechins%saponius%in vitro%antihyperlipdemic
以1 mmol·L-1油酸孵育HepG2细胞24 h,建立体外高脂模型,在细胞存活率实验(MTT法)的基础上,以脂质清除率为指标,评价茶黄素(TFs)、儿茶素(Cs)和绞股蓝皂苷(SPs)的体外降脂效果;并结合Box-Behnken响应曲面法(RSM)对3种物质体外联合降脂的浓度进行优化。结果表明:茶黄素、儿茶素和绞股蓝皂苷均能清除肝细胞内堆积的脂肪,若三者同时作用,协同效果明显,能起到防治高血脂症的作用。
以1 mmol·L-1油痠孵育HepG2細胞24 h,建立體外高脂模型,在細胞存活率實驗(MTT法)的基礎上,以脂質清除率為指標,評價茶黃素(TFs)、兒茶素(Cs)和絞股藍皂苷(SPs)的體外降脂效果;併結閤Box-Behnken響應麯麵法(RSM)對3種物質體外聯閤降脂的濃度進行優化。結果錶明:茶黃素、兒茶素和絞股藍皂苷均能清除肝細胞內堆積的脂肪,若三者同時作用,協同效果明顯,能起到防治高血脂癥的作用。
이1 mmol·L-1유산부육HepG2세포24 h,건입체외고지모형,재세포존활솔실험(MTT법)적기출상,이지질청제솔위지표,평개다황소(TFs)、인다소(Cs)화교고람조감(SPs)적체외강지효과;병결합Box-Behnken향응곡면법(RSM)대3충물질체외연합강지적농도진행우화。결과표명:다황소、인다소화교고람조감균능청제간세포내퇴적적지방,약삼자동시작용,협동효과명현,능기도방치고혈지증적작용。
The antihyperlipdemic activity in vitro of theaflavins(TFs), catechins(Cs) and saponius(SPs) were assessed in lipid clearance rate in the model of HepG2 steatosis by using the admini stration with 1 mmol·L-1 oleic acid(OA) in 24 h. Based on the cell viability test(MTT value), lipid clearance was used to evaluate the effect of TFs, Cs and SPs on HepG2 steatosis. The response surface methodology based on a Box-Behnken experimental design was used to optimize the density of the three extractions for combined lipid-lowering in vitro. Results indicated that TFs, Cs and SPs showed a significant inhibitory effect on TG accumulation in HepG2 cells. The ability of lipid-lowering was significant promoted when the three extractions acted on HepG2 at the same time. TFs, Cs and SPs could showed the prevention and curing effect of hyperlipidemia.