茶叶科学
茶葉科學
다협과학
2014年
4期
371-380
,共10页
陈春梅%马春雷%马建强%刘声传%陈亮
陳春梅%馬春雷%馬建彊%劉聲傳%陳亮
진춘매%마춘뢰%마건강%류성전%진량
茶树%叶绿体基因组%高通量测序%亲缘关系
茶樹%葉綠體基因組%高通量測序%親緣關繫
다수%협록체기인조%고통량측서%친연관계
tea plant%chloroplast genome%high-throughput sequencing%genetic relationship
叶绿体基因组在物种鉴定、系统进化分析及亲缘关系研究等领域应用前景广阔。本文应用 Illumina 高通量测序技术对龙井43（Camellia sinensis cv. Longjing 43）的叶绿体全基因组进行测序；利用叶绿体trnL-trnF序列研究茶树及其近缘植物的亲缘关系。结果表明，龙井43叶绿体基因组全长为157096 bp，反向互补重复区（Inverted repeat, IR）为26080 bp，小单拷贝区（Small single copy region, SSC）、大单拷贝区（Large single copy region, LSC）分别为18283 bp、86653 bp。共注释叶绿体基因133个，其中蛋白编码基因86个，rRNA基因8个，tRNA基因39个。对所选植物的trnL-trnF序列进行比对，序列长度变异范围为481~501 bp，序列最长为岔河大茶，最短为金花茶，基于此序列构建亲缘关系树，山茶属茶组植物聚成一个组。研究结果对茶树优良品种培育及山茶属植物亲缘关系的研究具有重要价值。
葉綠體基因組在物種鑒定、繫統進化分析及親緣關繫研究等領域應用前景廣闊。本文應用 Illumina 高通量測序技術對龍井43（Camellia sinensis cv. Longjing 43）的葉綠體全基因組進行測序；利用葉綠體trnL-trnF序列研究茶樹及其近緣植物的親緣關繫。結果錶明，龍井43葉綠體基因組全長為157096 bp，反嚮互補重複區（Inverted repeat, IR）為26080 bp，小單拷貝區（Small single copy region, SSC）、大單拷貝區（Large single copy region, LSC）分彆為18283 bp、86653 bp。共註釋葉綠體基因133箇，其中蛋白編碼基因86箇，rRNA基因8箇，tRNA基因39箇。對所選植物的trnL-trnF序列進行比對，序列長度變異範圍為481~501 bp，序列最長為岔河大茶，最短為金花茶，基于此序列構建親緣關繫樹，山茶屬茶組植物聚成一箇組。研究結果對茶樹優良品種培育及山茶屬植物親緣關繫的研究具有重要價值。
협록체기인조재물충감정、계통진화분석급친연관계연구등영역응용전경엄활。본문응용 Illumina 고통량측서기술대룡정43（Camellia sinensis cv. Longjing 43）적협록체전기인조진행측서；이용협록체trnL-trnF서렬연구다수급기근연식물적친연관계。결과표명，룡정43협록체기인조전장위157096 bp，반향호보중복구（Inverted repeat, IR）위26080 bp，소단고패구（Small single copy region, SSC）、대단고패구（Large single copy region, LSC）분별위18283 bp、86653 bp。공주석협록체기인133개，기중단백편마기인86개，rRNA기인8개，tRNA기인39개。대소선식물적trnL-trnF서렬진행비대，서렬장도변이범위위481~501 bp，서렬최장위차하대다，최단위금화다，기우차서렬구건친연관계수，산다속다조식물취성일개조。연구결과대다수우량품충배육급산다속식물친연관계적연구구유중요개치。
Chloroplast genome sequences have comprehensive application prospects in species identification, phylogeny analyses and transgenic breeding. The complete chloroplast genome of Camellia sinensis cv. Longjing 43 was sequenced using Illumina sequencing technology. The study of genetic relationship for tea plant and its closely related species based on chloroplast DNA sequences trnL-trnF. The results showed that the chloroplast genome of‘Longjing 43’ was found to be 157 096 bp in length which included a pair of inverted repeats (IRs) of 26 080 bp, separated by a small singlecopy region of 18 283 bp and a large single-copy region (LSC) of 86 653 bp. A total of 133 predicted genes including 86 protein-coding genes, 8 ribosomal RNA genes and 39 tRNA genes were identified. Sequence alignment of trnL-trnF for Plants were selected, the sequence of trnL-trnF ranged from 481 bp to 501 bp in length, the longest length of trnL-trnF region was 501 bp in C. tachangensis, the shortest was 481 bp in C. nitidissima. Phylogenetic analysis showed that all of the Sect. Thea being organized in the same clade. The results will play an important role for tea breeding and phylogenetic relationship study of tea plant and other Camellia species.
