CAJ | 학술논문

为了揭示星斑川鲽(Platichthys stellatus)♀×大菱鲆(Scophthalmus maximus)♂杂交后代的种质遗传属性，作者采用线粒体(mt)DNA COI基因片段和控制区(D-loop)序列对星斑川鲽、大菱鲆及两种间的杂交后代(星斑川鲽♀×大菱鲆♂)遗传特性进行研究。研究结果显示，基于mtDNA COI和D-loop序列结果显示星斑川鲽和大菱鲆的种间遗传距离分别为22.9%和41.2%，相似的研究结果显示星斑川鲽♀×大菱鲆♂杂交后代与大菱鲆的遗传距离分别为22.9%和41.2%，遗传差异极其显著。而基于mtDNA D-loop序列结果显示星斑川鲽♀×大菱鲆♂杂交后代与星斑川鲽的遗传距离仅为0.4%，并且在mtDNA COI基因片段上两者序列完全一致，杂交后代在线粒体DNA上呈现明显的偏母遗传。
위료게시성반천접(Platichthys stellatus)♀×대릉평(Scophthalmus maximus)♂잡교후대적충질유전속성，작자채용선립체(mt)DNA COI기인편단화공제구(D-loop)서렬대성반천접、대릉평급량충간적잡교후대(성반천접♀×대릉평♂)유전특성진행연구。연구결과현시，기우mtDNA COI화D-loop서렬결과현시성반천접화대릉평적충간유전거리분별위22.9%화41.2%，상사적연구결과현시성반천접♀×대릉평♂잡교후대여대릉평적유전거리분별위22.9%화41.2%，유전차이겁기현저。이기우mtDNA D-loop서렬결과현시성반천접♀×대릉평♂잡교후대여성반천접적유전거리부위0.4%，병차재mtDNA COI기인편단상량자서렬완전일치，잡교후대재선립체DNA상정현명현적편모유전。
Partial COI gene fragments and D-loop sequences of mitochondrial DNA were used to estimate genetic variability inPlatichthys stellatus,Scophthalmus maximus and their hybrids (P. stellatus♀×S. maximus♂). Based on the analyses of the mtDNA COI gene and D-loop fragments, the genetic distances (D) betweenP. stellatusand S. maximus were 22.9% and 41.2% respectively. Similar result was obtained between the hybrids (P. stellatus♀×S. maximus♂) andS. maximus, which showed significant differentiation between hybrids and male parent. A very low differentiation (D= 0.4%) was observed between the hybrid and female parent based on the mtDNA D-loop sequences, and no genetic differentiation was found between them based on the mtDNA COI gene sequences. According to the above results, the hybrids (P. stellatus♀×S. maximus♂) showed strict maternal inheritance in mtDNA genome.