CAJ | 학술논문

目的：建立高效毛细管电泳法测定保和丸中橙皮苷与连翘苷含量的方法。方法：采用区带毛细管电泳法，电泳条件为：未涂层石英毛细管柱（75 cm×50μm），运行缓冲液30 mmol·L-1硼砂溶液（含8%乙腈，pH9.64），压力进样50 kPa×20 s ,电压20 kV ，柱温20℃，检测波长254 nm。两次进样间用0.1 mol·L-1氢氧化钠和缓冲液分别冲洗10 min。结果：样品中橙皮苷与连翘苷均得到了很好的分离，其中橙皮苷与连翘苷分别在0.10~2.40 mg·mL-1（r=0.9994），0.07~0.84 mg·mL-1（r=0.9992）范围内呈良好线性关系，平均回收率分别为99.85%，99.16%，RSD 分别为2.34%，2.78%。结论：该方法简便、灵敏，在一定程度上弥补了传统方法的不足，适用于保和丸中橙皮苷与连翘苷的含量测定。
목적：건립고효모세관전영법측정보화환중등피감여련교감함량적방법。방법：채용구대모세관전영법，전영조건위：미도층석영모세관주（75 cm×50μm），운행완충액30 mmol·L-1붕사용액（함8%을정，pH9.64），압력진양50 kPa×20 s ,전압20 kV ，주온20℃，검측파장254 nm。량차진양간용0.1 mol·L-1경양화납화완충액분별충세10 min。결과：양품중등피감여련교감균득도료흔호적분리，기중등피감여련교감분별재0.10~2.40 mg·mL-1（r=0.9994），0.07~0.84 mg·mL-1（r=0.9992）범위내정량호선성관계，평균회수솔분별위99.85%，99.16%，RSD 분별위2.34%，2.78%。결론：해방법간편、령민，재일정정도상미보료전통방법적불족，괄용우보화환중등피감여련교감적함량측정。
This study was aimed to establish a HPCE method for the content determination of hesperidin and phillyrin in Bao-He-W an (BHW). Fused silica capillary (75 cm í 50 μm) was employed and 30 mmol·L-1 borax so-lution (8% acetonitrile, pH9.64) was served as the running buffer. Other conditions were as follows: electrokinetic injection was 50 kPa í 20 s; analytical voltage was 20 kV; temperature was 20℃; and detection wavelength was 254 nm. The silica capillary was flushed with 0.1 mol·L-1 sodium hydrate and the running buffer for 10 min before each injection, respectively. The results showed that the linearity of hesperidin was in the range of 0.10~2.40 mg·mL-1 (r=0.999 4), the average recovery was 99.85% and RSD=2.34%. The phillyrin was in the range of 0.07~0.84 mg·mL-1 (r=0.999 2), the average recovery was 99.16% and RSD=2.78%. It was concluded that the method was rapid and sensitive. It can be used for the quality control of content determination of hesperidin and phillyrin in BHW.