CAJ | 학술논문

目的：建立黔产一枝黄花药材HPLC指纹图谱。方法：采用高效液相色谱法，以Diamonsil C18（200 mm×4.6 mm，5μm）为色谱柱，乙腈-0.2%磷酸溶液为流动相，梯度洗脱，检测波长为282 nm，流速1.0 mL·min-1，柱温25℃，分析了10批不同产地一枝黄花药材指纹图谱。结果：10批一枝黄花药材中有13个共有峰，经相似度计算，整体相似度良好。结论：该方法稳定、重复性好，可用于黔产一枝黄花药材的质量控制。
목적：건립검산일지황화약재HPLC지문도보。방법：채용고효액상색보법，이Diamonsil C18（200 mm×4.6 mm，5μm）위색보주，을정-0.2%린산용액위류동상，제도세탈，검측파장위282 nm，류속1.0 mL·min-1，주온25℃，분석료10비불동산지일지황화약재지문도보。결과：10비일지황화약재중유13개공유봉，경상사도계산，정체상사도량호。결론：해방법은정、중복성호，가용우검산일지황화약재적질량공제。
This study was aimed to establish the fingerprints of Qian Solidaginis Herba by HPLC. The fingerprints of Qian Solidaginis Herba were built by using Diamonsil C18 (200 mm í 4.6 mm, 5 μm) column and acetonitrile -0.2%H3PO4 aqueous solution in gradient as mobile phase. The flow rate was 1.0 mL·min-1. Detecting wavelength was set at 282 nm. The total 10 batches of Qian Solidaginis Herba samples and their adulterants were analyzed. The results showed that 13 peaks were identified as the fingerprints of Qian Solidaginis Herba. Under chromatographic conditions mentioned above. It was concluded that the HPLC fingerprints method was found to have satisfactory accuracy, sta-bility and reproducibility, which was a potential method for the quality control of Qian Solidaginis Herba.