世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
7期
1542-1546
,共5页
核磁共振%小青龙汤证%大鼠%尿液%代谢组学
覈磁共振%小青龍湯證%大鼠%尿液%代謝組學
핵자공진%소청룡탕증%대서%뇨액%대사조학
Nuclear magnetic resonance%syndrome suitable for Small Qing-Long Decoction%rats%urine%metabonomics
目的：运用代谢组学核磁共振技术找出小青龙汤证大鼠尿液中的生物标志物，为小青龙汤证的临床诊断和治疗提供客观依据。方法：对空白对照组、模型组和小青龙汤组大鼠的尿液进行核磁共振氢谱检测，经MestRenova预处理和积分后，再用SIMCA-P+进行偏最小二乘法判别分析，得到相应的散点图和载荷图，进行比较后找出差异性代谢物。结果：与空白对照组比较，模型组中肌酸酐、胍基乙酸、三甲胺、牛磺酸、尿囊素等代谢物含量升高，琥珀酸、2-酮戊二酸、乳酸、甘氨酸、甲基烟碱等代谢物含量降低。用药后小青龙汤组中肌酸酐、三甲胺、尿囊素、牛磺酸与空白对照组比较明显升高，但与模型组比较明显降低；乳酸与空白对照组比较明显降低，但与模型组比较明显升高，胍基乙酸、2-酮戊二酸、N-甲基烟碱的改变则无统计学意义。结论：经分析可以看出，所找出的这些物质主要与能量代谢、炎症反应和免疫抵抗力有关，与能量代谢相关的物质变化尤为明显。
目的：運用代謝組學覈磁共振技術找齣小青龍湯證大鼠尿液中的生物標誌物，為小青龍湯證的臨床診斷和治療提供客觀依據。方法：對空白對照組、模型組和小青龍湯組大鼠的尿液進行覈磁共振氫譜檢測，經MestRenova預處理和積分後，再用SIMCA-P+進行偏最小二乘法判彆分析，得到相應的散點圖和載荷圖，進行比較後找齣差異性代謝物。結果：與空白對照組比較，模型組中肌痠酐、胍基乙痠、三甲胺、牛磺痠、尿囊素等代謝物含量升高，琥珀痠、2-酮戊二痠、乳痠、甘氨痠、甲基煙堿等代謝物含量降低。用藥後小青龍湯組中肌痠酐、三甲胺、尿囊素、牛磺痠與空白對照組比較明顯升高，但與模型組比較明顯降低；乳痠與空白對照組比較明顯降低，但與模型組比較明顯升高，胍基乙痠、2-酮戊二痠、N-甲基煙堿的改變則無統計學意義。結論：經分析可以看齣，所找齣的這些物質主要與能量代謝、炎癥反應和免疫牴抗力有關，與能量代謝相關的物質變化尤為明顯。
목적：운용대사조학핵자공진기술조출소청룡탕증대서뇨액중적생물표지물，위소청룡탕증적림상진단화치료제공객관의거。방법：대공백대조조、모형조화소청룡탕조대서적뇨액진행핵자공진경보검측，경MestRenova예처리화적분후，재용SIMCA-P+진행편최소이승법판별분석，득도상응적산점도화재하도，진행비교후조출차이성대사물。결과：여공백대조조비교，모형조중기산항、고기을산、삼갑알、우광산、뇨낭소등대사물함량승고，호박산、2-동무이산、유산、감안산、갑기연감등대사물함량강저。용약후소청룡탕조중기산항、삼갑알、뇨낭소、우광산여공백대조조비교명현승고，단여모형조비교명현강저；유산여공백대조조비교명현강저，단여모형조비교명현승고，고기을산、2-동무이산、N-갑기연감적개변칙무통계학의의。결론：경분석가이간출，소조출적저사물질주요여능량대사、염증반응화면역저항력유관，여능량대사상관적물질변화우위명현。
Through the map analysis of various metabolites in rat's urine, the biomarkers in syndrome suitable for Small Qing-Long Decoction (SQLD) were found out, which provided objective basis for the clinical diagnosis and treatment. Based on the rat's urine nuclear magnetic resonance (NMR) spectrum detection of the blank control group, the model group and the SQLD group, the data come from the map by MestRenova pretreatment and integration were analyzed using SIMCA-P+ by (PLS-DA). Then, the corresponding scatter diagram and diagram of loading were re-ceived and the differences of metabolites were found out by the comparison . The results showed that compared with the blank control group, contents of creatinine, guanidinoacetic acid, trimethylamine, taurine, allantoin metabolites in the model group had elevated. And the contents of succinic acid, 2-OG, lactic acid, glycine, methyl nicotine metabolite had decreased. After medication, contents of creatinine, trimethylamine, allantoin, taurine of SQLD group were still higher than the blank control group. However, the contents were reduced compared with the model group. The content of lactic acid was lower than the blank control group, but higher than the model group. There was no statistical significance on changes of guanidinoacetic acid, 2-OG, N-nornicotine. It was concluded that substances found were mainly related to energy metabolism, inflammation and immune resistance, which was especially related to energy metabolism change obviously.
