CAJ | 학술논문

目的：研究鹿茸不同部位水提液体外对细胞促增殖作用的差异。方法：以大鼠肾成纤维细胞NRK-49F作为模型，以MTT法检测鲜鹿茸上、中、下三部分水提液的促细胞增殖率，以BCA法检测样品蛋白浓度，SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）方法分析样品蛋白组成的差异。结果：上、中、下部鹿茸样品可溶性蛋白含量分别为17.89、16.04和6.89 mg·mL-1，从顶端到基部，鹿茸可溶性蛋白的含量依次降低。加样24 h时，鹿茸上部和中部样品分别在蛋白浓度800μg·mL-1和600μg·mL-1时达到最大促增殖率，分别为66.76%和64.36%，下部样品在1000μg·mL-1时达到最大促增殖率，为58.87%。作用48 h后，鹿茸上部和中部样品均在蛋白浓度800μg·mL-1时达到最大促增殖率，分别为219.56%和215.86%，下部样品在蛋白浓度1000μg·mL-1时达到最大促增殖率，为169.20%。鹿茸样品对细胞的增殖促进作用远大于10%胎牛血清。3个部位样品蛋白组成的SDS-PAGE图谱差异不大。结论：所有样品对细胞增殖均有促进作用，且呈现浓度依赖效应。不同部位样品主要蛋白组成差异不大。
목적：연구록용불동부위수제액체외대세포촉증식작용적차이。방법：이대서신성섬유세포NRK-49F작위모형，이MTT법검측선록용상、중、하삼부분수제액적촉세포증식솔，이BCA법검측양품단백농도，SDS-취병희선알응효전영（SDS-PAGE）방법분석양품단백조성적차이。결과：상、중、하부록용양품가용성단백함량분별위17.89、16.04화6.89 mg·mL-1，종정단도기부，록용가용성단백적함량의차강저。가양24 h시，록용상부화중부양품분별재단백농도800μg·mL-1화600μg·mL-1시체도최대촉증식솔，분별위66.76%화64.36%，하부양품재1000μg·mL-1시체도최대촉증식솔，위58.87%。작용48 h후，록용상부화중부양품균재단백농도800μg·mL-1시체도최대촉증식솔，분별위219.56%화215.86%，하부양품재단백농도1000μg·mL-1시체도최대촉증식솔，위169.20%。록용양품대세포적증식촉진작용원대우10%태우혈청。3개부위양품단백조성적SDS-PAGE도보차이불대。결론：소유양품대세포증식균유촉진작용，차정현농도의뢰효응。불동부위양품주요단백조성차이불대。
This study was aimed to explore differential effects of various sections of the velvet antler on promoting cell proliferation in vitro. The NRK-49F cell line from rat kidney fibroblasts was used as the cell model. The cell proliferation rates of the water extracts from the upper, middle and lower section of fresh velvet antler were measured by the MTT method. BCA method was used in the detection of protein concentration. The SDS-PAGE method was used in the analysis of difference composition of the sample protein. The results showed that soluble protein content of the upper, middle and lower section were 17.89, 16.04 and 6.89 mg·mL-1, respectively. From the top to the base, the soluble protein content of velvet antler was decreased. After 24 h treatment, when the protein concentration of the upper and middle section samples of the velvet antler were 800 μg·mL-1 and 600 μg·mL-1, the cell proliferation promoting rates reached the maximum, which were 66.76% and 64.36%, respectively. And when the lower section sample of the velvet antler was 1 000 μg·mL-1, the cell proliferation promoting rates reached the maximum, which was 58.87%. After 48 h treatment, when the upper and middle section samples of the velvet antler were 800μg·mL-1, the cell proliferation promoting rates reached the maximum, which were 219.56% and 215.86%, respectively. And when the lower section sample of the velvet antler was 1 000 μg·mL-1, the cell proliferation promoting rates reached the maximum, which was 169.20%. The velvet antler on the proliferation of cells was much better than the 10% fetal bovine serum. The figure of SDS-PAGE showed the slight difference in the protein composition of three part of the velvet antler. It was concluded that all samples had promoting effects on cell proliferation with concentration-depen-dent, and the main protein in different part of the velvet antler had minor differences.