CAJ | 학술논문

目的：研究茉莉酸甲酯对阳春砂挥发性萜类合成的影响，深入了解挥发性萜类生物合成的分子调控机制。方法：用不同浓度茉莉酸甲酯溶液喷施阳春砂的叶片和果实，用微波法提取果实中的挥发性萜类成分，应用气相色谱-质谱（GC-MS）联用技术检测挥发性萜类成分的变化，通过转录组测序构建cD-NA文库并进行生物信息学分析。结果：果皮和种子团中分别检测到20种和33种挥发性萜类成分，600μmol·L-1 MeJA喷果24 h后果皮和种子团中大部分挥发性萜类成分的积累量明显增加，如乙酸龙脑酯、樟脑和龙脑等；而200μmol·L-1 MeJA喷施叶片或果实对果皮及种子团中不同挥发性萜类含量的影响存在差异，且200μmol·L-1 MeJA喷施果实比喷施叶片更能提高种子团中挥发性萜类成分的积累量。此外，转录组测序共获得68168个Unigene，通过与公共数据库比对，共有48627个Unigene获得注释。对KEGG代谢通路的注释结果进行初步分析，发现挥发性萜类代谢密切相关的Unigene有208个，MYC2类转录因子（JA信号转导的重要调控因子）相关的Unigene有22个。结论：MeJA能有效调控阳春砂中挥发性萜类成分的代谢，转录组测序获得了多个与挥发性萜类合成相关的候选基因，为深入开展阳春砂挥发性萜类生物合成相关功能基因的发掘及调控研究提供了丰富的数据资源。
목적：연구말리산갑지대양춘사휘발성첩류합성적영향，심입료해휘발성첩류생물합성적분자조공궤제。방법：용불동농도말리산갑지용액분시양춘사적협편화과실，용미파법제취과실중적휘발성첩류성분，응용기상색보-질보（GC-MS）련용기술검측휘발성첩류성분적변화，통과전록조측서구건cD-NA문고병진행생물신식학분석。결과：과피화충자단중분별검측도20충화33충휘발성첩류성분，600μmol·L-1 MeJA분과24 h후과피화충자단중대부분휘발성첩류성분적적루량명현증가，여을산용뇌지、장뇌화용뇌등；이200μmol·L-1 MeJA분시협편혹과실대과피급충자단중불동휘발성첩류함량적영향존재차이，차200μmol·L-1 MeJA분시과실비분시협편경능제고충자단중휘발성첩류성분적적루량。차외，전록조측서공획득68168개Unigene，통과여공공수거고비대，공유48627개Unigene획득주석。대KEGG대사통로적주석결과진행초보분석，발현휘발성첩류대사밀절상관적Unigene유208개，MYC2류전록인자（JA신호전도적중요조공인자）상관적Unigene유22개。결론：MeJA능유효조공양춘사중휘발성첩류성분적대사，전록조측서획득료다개여휘발성첩류합성상관적후선기인，위심입개전양춘사휘발성첩류생물합성상관공능기인적발굴급조공연구제공료봉부적수거자원。
This study was aimed to reveal the effects and molecular regulation mechanism of methyl jasmonate (Me-JA) on volatile terpenoids from Amomum villosum Lour. After the leaves and fruits of A momum villosum Lour. were treated with different concentrations of MeJA, the volatile terpenoids of fresh fruits from A . villosum Lour. were ex-tracted with microwave method and analyzed by GC-MS. Then, leaves and fruits treated with MeJA were sequenced by Illumina. The transcriptome data was analyzed by bioinformatic methods. The results showed that there were 20 and 33 volatile terpenoids detected in peels and seed groups, respectively. Contents of volatile terpenoids in peels and seed groups were both improved after 600 μmol·L-1 MeJA treating fruits for 24 h, such as bornyl acetate, cam-phor, borneol, and etc. While 200 μmol·L-1 MeJA treating different parts for 24 h can regulate the biosynthesis of some volatile terpenoids in peels differently. And 200 μmol·L-1 MeJA treating fruits can improve the content of ma-jor volatile terpenoids in seed groups. A total of 68 168 unigenes were obtained with de novo assembly, and 48 627 unigenes were annotated after comparison with public protein databases. Analysis of functional annotation against KEGG database showed that there were 208 unigenes closely related with metabolism of volatile terpenoids and 22 u-nigenes related with MYC2 transcription factors. It was concluded that MeJA can effectively regulate the metabolism of volatile terpenoids from A . villosum Lour. There were a lot of candidate genes related with the biosynthesis of volatile terpenoids obtained by analyzing the transcriptome data which also provided a large amount of data for the discovery and regulation of functional genes related with the biosynthesis of volatile terpenoids from A . villosum Lour.